Anti-Mouse MAdCAM-1 (MECA-89) – Purified in vivo GOLDTM Functional Grade
Anti-Mouse MAdCAM-1 (MECA-89) – Purified in vivo GOLDTM Functional Grade
Product No.: M1420
Clone MECA-89 Target MADCAM-1 Formats AvailableView All Product Type Hybridoma Monoclonal Antibody Alternate Names Mucosal addressin cell adhesion molecule-1 Isotype Rat IgG2a κ Applications B , FA , FC , IF , IHC , IP , WB |
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Recommended Isotype Controls Recommended Isotype Controls Recommended Dilution Buffer Immunogen Mouse mesenteric and peripheral lymph node cells Product Concentration ≥ 5.0 mg/ml Endotoxin Level ≤ 1.0 EU/mg as determined by the LAL method Purity ≥95% by SDS Page ⋅ ≥95% monomer by analytical SEC Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. State of Matter Liquid Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Regulatory Status Research Use Only Country of Origin USA Shipping 2 - 8°C Wet Ice Additional Applications Reported In Literature ? FA IHC IF IP WB FC B Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity MECA-89 activity is directed against mouse MAdCAM-1. Background MAdCAM-1 is a cell adhesion leukocyte receptor expressed by mucosal venules that helps direct lymphocyte traffic into mucosal tissues and regulates the passage and retention of leukocytes1, 2. MAdCAM-1 binds integrin receptor α4β7 and L-selectin2, 3, 4. Two alternatively spliced isoforms of MAdCAM-1 exist5, both of which are capable of binding α4β72. MECA-89 was generated by immunizing Wistar rats with endothelial cells isolated from BALB/c mesenteric and peripheral lymph nodes 6. Immunohistological screening of hybridomas yielded two mAbs, MECA-367 and MECA-89, that stain high endothelial venules (HEVs) in mucosal lymphoid organs and Peyer’s patches, but not peripheral lymph nodes (axillary, brachial, popliteal, and inguinal). Immunofluorescence staining of high endothelial cells shows that both MECA-367 and MECA-89 react with antigens on the cell surface. The epitopes for MECA-367 and MECA-89 are distinct. MECA-367 recognizes the N-terminal immunoglobulin domain of MAdCAM-l, and MECA-89 recognizes the second immunoglobulin domain 4,5. MECA-89 reacts with the same vessels as MECA-367 and binds to isolated MECA-367 antigen; however, unlike MECA-367 it has no effect on lymphocyte binding 6. Additionally, MECA-89 has no effect on MAdCAM-1 binding to α4β7 in vitro 7, but L-selectin dependent adhesion is lost in the presence of MECA-89 4. Additionally, MECA-89 has no significant effect on activated cells, but all interactions are inhibited following subsequent injection of anti-α4 Fab fragments 4. In vivo, MECA-89 inhibits L-selectin-dependent rolling but not direct α4β7-dependent attachment of Mn2+ activated lymphocytes. Antigen Distribution MAdCAM-1 is a cell surface glycoprotein selectively expressed on high endothelial venules of mucosal lymphoid organs and Peyer’s patches as well as lamina propria venules. Ligand/Receptor Integrin a4ß7, CD62L NCBI Gene Bank ID UniProt.org Research Area Cell Adhesion . Cell Biology . Immunology Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Clone MECA-89 is a rat monoclonal antibody targeting mouse MAdCAM-1, commonly employed in various in vivo applications in mice. Here are some of the primary uses: Common In Vivo Applications of Clone MECA-89
Commonly used antibodies or proteins in the literature used alongside MECA-89 (an anti-MAdCAM-1 antibody) include:
Summary Table: Common Antibodies/Proteins Used with MECA-89
These combinations allow detailed dissection of vascular addressin and integrin roles in tissue-specific lymphocyte homing and inflammation, and are well established in immunology literature. The selection depends on the research focus (e.g., mucosal vs. peripheral lymphoid tissues, integrin blocking vs. mapping addressin expression). Key findings from scientific literature citing clone MECA-89 focus primarily on its use as a monoclonal antibody tool for immunological and histological studies, particularly in identifying and characterizing the expression and function of MAdCAM-1 (Mucosal Addressin Cell Adhesion Molecule-1) in mouse tissue. Essential context and supporting details:
Additional relevant information:
In summary, citations of clone MECA-89 in scientific literature emphasize its importance as a research tool for understanding MAdCAM-1-mediated immune processes in mice, with applications spanning basic immunology, lymphoid tissue development, and disease models. The dosing regimens of clone MECA-89, an anti-Mouse MAdCAM-1 antibody, vary across different mouse models depending on several factors, including the specific disease context, pharmacokinetic parameters, and intended pharmacodynamic effects. Unfortunately, direct and detailed information on MECA-89 dosing regimens in recent literature is limited, and most information is model-dependent, suggesting that dosages are tailored to the specific requirements of each study. In general, dosing regimens for antibodies in mouse models can be influenced by factors such as the target of the antibody, the disease being studied, the route of administration, and the frequency of dosing. For example, other antibodies used in mouse models often involve intraperitoneal or intravenous injections, with dosages ranging from micrograms to milligrams per mouse, depending on the application and the model used. While specific details for MECA-89 are not readily available, understanding the factors that influence dosing for similar antibodies can help in designing experiments. However, without specific data on MECA-89, researchers would need to rely on general principles or conduct preliminary studies to determine optimal dosages for their particular models. References & Citations1. https://www.uniprot.org/uniprotkb/Q61826/entry
2. Schiffer SG, Day E, Latanision SM, et al. Biochem Biophys Res Commun. 216(1):170-176. 1995. 3. Berlin C, Berg EL, Briskin MJ, et al. Cell. 74(1):185-195. 1993. 4. Bargatze RF, Jutila MA, Butcher EC. Immunity. 3(1):99-108. 1995. 5. Briskin MJ, McEvoy LM, Butcher EC. Nature. 363(6428):461-464. 1993. 6. Streeter PR, Berg EL, Rouse BT, et al. Nature. 331(6151):41-46. 1988. 7. Nakache M, Berg EL, Streeter PR, et al. Nature. 337(6203):179-181. 1989. Technical ProtocolsB FA  IF    Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
