Anti-Mouse MHC Class I (H-2Kd, H-2Dd) [Clone 34-1-2S] — Biotin

Spleen cells from (B6 X DBA/2) BDF1 mice

This Biotinylated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Liquid

This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.

Research Use Only

2 – 8° C Wet Ice

34-1-2S activity is directed against H-2D^d and H-2K^d MHC Class I antigens. 34-1-2S cross-reacts with K^b, s, r, q, and p. Binding to H-2K^b is weak. 34-1-2S does not cross-react with H-2^k and H-2^f.

H-2, the murine major histocompatibility complex (MHC), is composed of a diverse group of antigens divided into class I and II proteins that function in immune response¹. MHC class I molecules bind peptides generated by the degradation of cytosolic proteins and then display those peptides on the cell surface. Generally, these peptides are derived from normal metabolism, but they can also be derived from foreign proteins during viral infection or allotransplantation. When peptides are recognized as foreign, cytotoxic T lymphocytes specific to the MHC class I- peptide complex kill the presenting cell.

34-1-2S was generated by immunizing C3H mice with spleen cells from (B6 X DBA/2) F₁ mice². Spleen cells were fused with SP2/0.Ag14 of BALB/c origin. 34-1-2S is cytotoxic to strains of many haplotypes. Deglycosylated 34-1-2S, and its Fab fragments, have abrogated binding to murine FcγRs compared with untreated or full-length antibodies³.

34-1-2S is known to induce transfusion-related acute lung injury and is used to study its pathogenesis^3,4,5,6,7. Injection of 34-1-2S into BALB/c and other H-2^d haplotype mice causes an H-2^d–specific change in breathing accompanied by decreased blood oxygenation, increased lung water content, protein leakage into airways, and redistribution of blood neutrophils to the lungs⁶.

H-2K^d and D^d are present on all nucleated cells.

Peptides derived from normal protein catabolism or foreign proteins e.g., viral infection, allotransplantation

H-2K(D):P01902
H-2D(D):P01900

1 Yoshida R. Adv Immunol. 124:207-247. 2014.
2 Ozato K, Mayer NM, Sachs DH. Transplantation. 34(3):113-120. 1982.
3 Zeeuw van der Laan EAN, van der Velden S, Bentlage AEH, et al. Blood Adv. 4(16):3875-3885. 2020.
4 Looney MR, Su X, Van Ziffle JA, et al. J Clin Invest. 116(6):1615-1623. 2006.
5 Looney MR, Nguyen JX, Hu Y, et al. J Clin Invest. Nov;119(11):3450-3461. 2009.
6 Strait RT, Hicks W, Barasa N, et al. J Exp Med. 208(12):2525-2544. 2011.
7 van der Velden S, van Osch TLJ, Seghier A, et al. Blood. 143(1):79-91. 2024.