Anti-Mouse/Rat CD71 [Clone OX-26] — Purified in vivo GOLD™ Functional Grade
Anti-Mouse/Rat CD71 [Clone OX-26] — Purified in vivo GOLD™ Functional Grade
Product No.: C853
Clone OX-26 Target CD71 Formats AvailableView All Product Type Monoclonal Antibody Isotype Mouse IgG2a Applications FC , IHC , in vivo |
Antibody DetailsProduct DetailsReactive Species Mouse ⋅ Rat Host Species Mouse Recommended Isotype Controls Recommended Dilution Buffer Product Concentration ≥ 5.0 mg/ml Endotoxin Level <1.0 EU/µg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.0-7.5, 0.005% pS80 stabilizing buffer, with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco FC IHC Additional Applications Reported In Literature ? IHC FC Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone OX-26 reacts with rat CD71 (TfR1). Background CD71 is a transferrin receptor on the cell surface that mediates the uptake of transferrin-iron complexes, thereby mediating cellular proliferation1. CD71 is a homodimeric glycoprotein containing 760 amino acids and it binds to diferric transferrin2. Binding by diferric transfferin causes CD71 to be internalized, and diferric iron is subsequently released3. CD71 is highly expressed on malignant cells, as iron uptake is necessary for aberrant cell proliferation4. Antigen Distribution CD71 is expressed on the surface of proliferating cells, reticulocytes, and erythroid precursors. UniProt.org Research Area Immunology Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Clone OX-26 is a monoclonal antibody that targets the transferrin receptor (CD71), primarily recognizing rat CD71, but variants and formulations have also been engineered to cross-react with mouse CD71. The antibody is widely used in experimental settings involving rats, and in some cases, mice. Common in vivo applications of clone OX-26 in mice include:
Key technical constraints and notes:
Summary Table: OX-26 in Mouse In Vivo Applications
In summary, OX-26 is mainly used for rat studies, with some application in mice when cross-reactivity is adequate—especially for flow cytometry and studies of proliferative cells. For targeted drug delivery across the mouse BBB, clone 8D3 or engineered mouse-reactive antibodies are much more effective. In the literature, several other antibodies or proteins are commonly used alongside OX-26 for various research purposes:
The clone OX-26 antibody is a well-characterized mouse monoclonal antibody that specifically targets the rat CD71 (transferrin receptor protein 1, TfR1). Key findings from scientific literature regarding clone OX-26 include:
The search results do not provide specific information on the dosing regimens of clone OX-26 across different mouse models. However, they highlight the use of OX-26, a monoclonal antibody targeting the transferrin receptor, in delivering substances across the blood-brain barrier in rat models rather than mouse models specifically. For mouse models, dosing regimens can vary significantly based on the specific research goals, model characteristics, and the drugs or substances being studied. For example, dosing strategies for other compounds like oxaliplatin in mouse models have been documented, where the total human equivalent dose (THED) varies from 0.04 to 10 mg/kg/day, and cumulative doses range from 3 to 30 mg/kg. However, this information is not directly applicable to OX-26 dosing. If OX-26 is used in mouse models, the dosing would likely depend on factors such as the specific application (e.g., drug delivery or immunological studies), the model's genetic background, and whether the model is used for studying neurological or other diseases. Researchers typically consult guidelines for in vivo antibody dosing in mouse models to optimize dosing strategies. In summary, while there is no specific information on OX-26 dosing regimens in mouse models, researchers would typically need to design and optimize these regimens based on the specific requirements of their study model. References & Citations1. Aisen P. Int J Biochem Cell Biol. Nov;36(11):2137-43. 2004.
2. Lawrence CM, Ray S, Babyonyshev M, et. al. Science. Oct 22;286(5440):779-82. 1999. 3. Marsee DK, Pinkus GS, Yu H. Am J Clin Pathol. Sep;134(3):429-35. 2010. 4. Candelaria PV, Leoh LS, Penichet ML, Daniels-Wells TR. Front Immunol. Mar 17;12:607692. 2021 5. Jiménez E, Sacedón R, Vicente A, et. al. J Immunol. May 15;168(10):5005-13. 2002. 6. Fabriek BO, Polfliet MM, Vloet RP, et. al. Blood. Jun 15;109(12):5223-9. 2007. Technical Protocols   Certificate of Analysis |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
