Anti-Mouse/Rat CD71 – Purified in vivo GOLD™ Functional Grade

Anti-Mouse/Rat CD71 – Purified in vivo GOLD™ Functional Grade

Product No.: C853

[product_table name="All Top" skus="C371"]

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Clone
OX-26
Target
CD71
Formats AvailableView All
Product Type
Monoclonal Antibody
Isotype
IgG2a
Applications
FC
,
IHC
,
in vivo

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Select Product Size
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Antibody Details

Product Details

Reactive Species
Mouse/Rat
Host Species
Mouse
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
<1.0 EU/µg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.0-7.5, 0.005% pS80 stabilizing buffer, with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
FC
IHC
Additional Applications Reported In Literature ?
IHC
FC
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone OX-26 reacts with rat CD71 (TfR1).
Background
CD71 is a transferrin receptor on the cell surface that mediates the uptake of transferrin-iron complexes, thereby mediating cellular proliferation1. CD71 is a homodimeric glycoprotein containing 760 amino acids and it binds to diferric transferrin2. Binding by diferric transfferin causes CD71 to be internalized, and diferric iron is subsequently released3. CD71 is highly expressed on malignant cells, as iron uptake is necessary for aberrant cell proliferation4.
Antigen Distribution
CD71 is expressed on the surface of proliferating cells, reticulocytes, and erythroid precursors.

Antigen Details

Research Area
Immunology

References & Citations

1. Aisen P. Int J Biochem Cell Biol. Nov;36(11):2137-43. 2004.
2. Lawrence CM, Ray S, Babyonyshev M, et. al. Science. Oct 22;286(5440):779-82. 1999.
3. Marsee DK, Pinkus GS, Yu H. Am J Clin Pathol. Sep;134(3):429-35. 2010.
4. Candelaria PV, Leoh LS, Penichet ML, Daniels-Wells TR. Front Immunol. Mar 17;12:607692. 2021
5. Jiménez E, Sacedón R, Vicente A, et. al. J Immunol. May 15;168(10):5005-13. 2002.
6. Fabriek BO, Polfliet MM, Vloet RP, et. al. Blood. Jun 15;109(12):5223-9. 2007.
Flow Cytometry
IHC
in vivo Protocol

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.