Anti-Mouse/Rat CD71 – Purified in vivo GOLD™ Functional Grade

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.0-7.5, 0.005% pS80 stabilizing buffer, with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.

Next Day 2-8°C

Clone OX-26 reacts with rat CD71 (TfR1).

CD71 is a transferrin receptor on the cell surface that mediates the uptake of transferrin-iron complexes, thereby mediating cellular proliferation¹. CD71 is a homodimeric glycoprotein containing 760 amino acids and it binds to diferric transferrin². Binding by diferric transfferin causes CD71 to be internalized, and diferric iron is subsequently released³. CD71 is highly expressed on malignant cells, as iron uptake is necessary for aberrant cell proliferation⁴.

CD71 is expressed on the surface of proliferating cells, reticulocytes, and erythroid precursors.

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