Anti-Mouse/Rat CD71 – Purified in vivo GOLD™ Functional Grade

Pricing & Details

Product No.C853
Clone
OX-26
Protein
CD71
Formats AvailableView All
Product Type
Monoclonal Antibody
Isotype
Mouse
IgG2a κ
Applications
FC
,
IHC
Prod No.
Size
Price
Avail.
Qty
Add to cart
C853-1.0mg
1.0 mg
$105.00
In stock
Max:
Min: 1
Step: 1
C853-5.0mg
5.0 mg
$355.00
In stock
Max:
Min: 1
Step: 1
C853-25mg
25 mg
$965.00
In stock
Max:
Min: 1
Step: 1
C853-50mg
50 mg
$1,595.00
In stock
Max:
Min: 1
Step: 1
C853-100mg
100 mg
$2,300.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Mouse/Rat
Host Species
Mouse
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
<1.0 EU/µg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
FC
IHC
Other Applications Reported In Literature ?
IHC
FC
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone OX-26 reacts with rat CD71 (TfR1).
Antigen Distribution
CD71 is expressed on the surface of proliferating cells, reticulocytes, and erythroid precursors.
Background
CD71 is a transferrin receptor on the cell surface that mediates the uptake of transferrin-iron complexes, thereby mediating cellular proliferation1. CD71 is a homodimeric glycoprotein containing 760 amino acids and it binds to diferric transferrin2. Binding by diferric transfferin causes CD71 to be internalized, and diferric iron is subsequently released3. CD71 is highly expressed on malignant cells, as iron uptake is necessary for aberrant cell proliferation4.

Antigen Details

Protein
PubMed
Research Area
Immunology

References & Citations

1. Aisen P. Int J Biochem Cell Biol. Nov;36(11):2137-43. 2004.
2. Lawrence CM, Ray S, Babyonyshev M, et. al. Science. Oct 22;286(5440):779-82. 1999.
3. Marsee DK, Pinkus GS, Yu H. Am J Clin Pathol. Sep;134(3):429-35. 2010.
4. Candelaria PV, Leoh LS, Penichet ML, Daniels-Wells TR. Front Immunol. Mar 17;12:607692. 2021
5. Jiménez E, Sacedón R, Vicente A, et. al. J Immunol. May 15;168(10):5005-13. 2002.
6. Fabriek BO, Polfliet MM, Vloet RP, et. al. Blood. Jun 15;109(12):5223-9. 2007.
Flow Cytometry
IHC

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.