Anti-Mouse TCR β chain – Purified in vivo PLATINUM™ Functional Grade
Anti-Mouse TCR β chain – Purified in vivo PLATINUM™ Functional Grade
Product No.: T772
Clone H57-597 Protein TCR beta Formats AvailableView All Product Type Monoclonal Antibody Alternate Names TCR-β chain, TCR-β, β-TCR Isotype Armenian Hamster IgG Applications Costim , Depletion , FC , IHC FF , IHC FFPE , in vivo , IP , PhenoCycler® |
Antibody DetailsProduct DetailsReactivity Species Mouse Host Species Armenian Hamster Immunogen Affinity purified TCR from mouse DO-11.10 cells Product Concentration ≥ 5.0 mg/ml Endotoxin Level ≤ 0.5 EU/mg as determined by the LAL method Purity ≥98% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Pathogen Testing To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUMTM antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2892982 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this TCR β chain (clone H57-597) antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. Additional Applications Reported In Literature ? Costim Depletion IP IHC FF IHC FFPE Additional Reported Applications For Relevant Conjugates ? PhenoCycler-Fusion (CODEX)® Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Clone H57-597 recognizes an epitope on the constant region of mouse TCRβ. Clone H57-597 does not cross-react with γδ T cells. Antigen Distribution TCRβ is expressed on αβ thymocytes, peripheral T cells, and NKT cells. Background TCRβ antibody, clone H57-597, recognizes the beta chain of the T cell receptor (TCRβ), expressed on αβ thymocytes and T lymphocytes, as well as natural killer T (NKT) cells1. TCRβ is a transmembrane protein that consists of an amino-terminal variable region, a constant region, and a stalk segment covalently linked to the TCR alpha chain (TCRα) via a disulfide bond2. TCRα/β does not contain signaling domains and is instead noncovalently associated with the CD3 complex (TCR-CD3), essential for TCR signaling3,4. TCRα/β recognizes antigens bound to major histocompatibility complex (MHC) molecules, resulting in phosphorylation of CD3 ITAMs and subsequent T cell activation, proliferation, and survival5,6. Antigen DetailsProtein Ligand/Receptor Peptide bound-MHC class I and II Function Antigen recognition, T cell activation PubMed NCBI Gene Bank ID UniProt.org References & Citations1. Krijgsman D, et al. (2018) Front Immunol. 9:367 2. Clevers H, et al. (1988) Annu Rev Immunol. 6:629-62 3. Kuhns MS, Badgandi HB. (2012) Immunol Rev. 250(1):120-43 4. Wucherpfennig KW, et al. (2010) Cold Spring Harb Perspect Biol. 2(4):a005140 5. Samelson LE. (2002) Annu Rev Immunol. 20:371-94 6. Borroto A, et al. (2014) Immunol Lett. 161(1):113-7 Technical Protocols |
Formats Available
Prod No. | Description |
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T663 |
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T668 |
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T669 |
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T670 |
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T671 |
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T664 |
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T900 |
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T662 |
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T648 |
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T666 |
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T665 |
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T772 |
