Anti-Mouse TCR β chain – Purified in vivo PLATINUM™ Functional Grade

Anti-Mouse TCR β chain – Purified in vivo PLATINUM™ Functional Grade

Product No.: T772

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Clone
H57-597
Target
TCR beta
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
TCR-β chain, TCR-β, β-TCR
Isotype
IgG
Applications
Costim
,
Depletion
,
FC
,
IHC FF
,
IHC FFPE
,
in vivo
,
IP
,
PhenoCycler®

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Select Product Size
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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Armenian Hamster
Immunogen
Affinity purified TCR from mouse DO-11.10 cells
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
≤ 0.5 EU/mg as determined by the LAL method
Purity
≥98% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM<sup>TM</sup> antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this TCR β chain (clone H57-597) antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Additional Applications Reported In Literature ?
Costim
Depletion
IP
IHC FF
IHC FFPE
Additional Reported Applications For Relevant Conjugates ?
PhenoCycler-Fusion (CODEX)®
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone H57-597 recognizes an epitope on the constant region of mouse TCRβ. Clone H57-597 does not cross-react with γδ T cells.
Background
TCRβ antibody, clone H57-597, recognizes the beta chain of the T cell receptor (TCRβ), expressed on αβ thymocytes and T lymphocytes, as well as natural killer T (NKT) cells1. TCRβ is a transmembrane protein that consists of an amino-terminal variable region, a constant region, and a stalk segment covalently linked to the TCR alpha chain (TCRα) via a disulfide bond2. TCRα/β does not contain signaling domains and is instead noncovalently associated with the CD3 complex (TCR-CD3), essential for TCR signaling3,4. TCRα/β recognizes antigens bound to major histocompatibility complex (MHC) molecules, resulting in phosphorylation of CD3 ITAMs and subsequent T cell activation, proliferation, and survival5,6.
Antigen Distribution
TCRβ is expressed on αβ thymocytes, peripheral T cells, and NKT cells.

Antigen Details

Ligand/Receptor
Peptide bound-MHC class I and II
Function
Antigen recognition, T cell activation
PubMed
NCBI Gene Bank ID

References & Citations

1. Krijgsman D, et al. (2018) Front Immunol. 9:367
2. Clevers H, et al. (1988) Annu Rev Immunol. 6:629-62
3. Kuhns MS, Badgandi HB. (2012) Immunol Rev. 250(1):120-43
4. Wucherpfennig KW, et al. (2010) Cold Spring Harb Perspect Biol. 2(4):a005140
5. Samelson LE. (2002) Annu Rev Immunol. 20:371-94
6. Borroto A, et al. (2014) Immunol Lett. 161(1):113-7
Costim
Depletion
Flow Cytometry
IHC FF
IHC FFPE
in vivo Protocol
Immunoprecipitation Protocol
PhenoCycler®

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.