Anti-Myc (phospho-Thr358) Antibody (43035)
Anti-Myc (phospho-Thr358) Antibody (43035)
Product No.: 43035
Data
Immunoblot: Detection of Myc (phosphoThr358) in extracts of HT29 cells
untreated or treated with UV.
Immunofluorescence: Detection of Myc (phosphoThr358 in methanol-fixed HeLa
cells
Immunohistochemistry: Detection of Myc (phosphoThr358) in paraffin-embedded
human breast carcinoma tissue. - -
- -
Antibody DetailsProduct DetailsReactivity Species Human Host Species Rabbit Immunogen Peptide sequence that includes phosphorylation site of threonine 358 (R-R-T(p)-H-N) derived from human Myc and conjugated to KLH. Product Concentration 1.0 mg/ml Formulation PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. State of Matter Liquid Product Preparation Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide Storage and Handling This antibody is stable for at least one (1) year at -20°C. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~60kDa is detected.
Immunohistochemistry: use at dilution of 1:50-1:100. Immunofluorescence: use at 1:100-1:200. These are recommended working dilutions. End user should determine optimal dilutions for their applications. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Rabbit Polyclonal Antibody specific to Myc (phospho-Thr358) Background Myc (c-Myc) is a regulator gene that codes for transcription factor Myc. This protein is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. A mutated version of Myc is found in many cancers which causes Myc to be constitutively expressed. Antigen DetailsResearch Area Phosphospecific Antibodies References & CitationsTechnical ProtocolsIF   |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
