Anti-NFkB-p65 (Phospho-Thr435)
Anti-NFkB-p65 (Phospho-Thr435)
Product No.: 43012
Data
Immunoblot: Detection of NFκB-p65 (phosphoThr435) in HeLa cell extracts
untreated or treated with
TNFα+CA.
Immunofluorescence: Detection of NFκB-p65 (phosphoThr435) in methanol-fixed HeLa
cells.
Immunohistochemistry: Detection of NFκB-p65 (phosphoThr435) in paraffin-embedded
human breast carcinoma tissue. - -
- -
Antibody DetailsProduct DetailsReactivity Species Human ⋅ Mouse ⋅ Rat Host Species Rabbit Immunogen Peptide sequence that includes phosphorylation site of threonine 435 (E-G-T(p)-L-S) derived from human NFkappaB-p65 and conjugated to KLH. Product Concentration 1.0 mg/ml Formulation PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. State of Matter Liquid Product Preparation Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide Storage and Handling This antibody is stable for at least one (1) year at -20°C. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Western blotting: use at dilution of 1:500-1:1,000. A band of ~65kDa is detected.
Immunohistochemistry: use at dilution of 1:50-1:100. Immunofluorescence: use at dilution of 1:100-1:200. These are recommended working dilutions. End user should determine optimal dilutions for their applications. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Rabbit Polyclonal Antibody specific to NFkB-p65 (Phospho-Thr435) Background NFkappaB is a transcription factor found in almost all cell types and is involved in many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NFkappaB is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. such as p65-p50 and p65-c-Rel complexes. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. Antigen DetailsResearch Area Phosphospecific Antibodies References & CitationsTechnical ProtocolsIF   |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
