Anti-Osteocalcin Antibody

Product No.: 50116

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Clone
OCG3
Target
Osteocalcin
Formats AvailableView All
Product Type
Monoclonal
Alternate Names
Bone Gla protein, BGP, γ-carboxyglutamic acid-containing protein
Isotype
IgG3
Applications
ELISA
,
IHC

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Select Product Size

Data

Immunohistochemistry: 1-10ug/ml, frozen or paraffin-embedded tissue after proteinase K (0.4m/ml) antigen retrieval. Chondroma: Human bone tumor. Synovial Sarcoma: human osteosarcoma.
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Antibody Details

Product Details

Reactivity Species
Bovine
Goat
Human
Porcine
Rabbit
Rat
Sheep
Host Species
Mouse
Immunogen
Bovine osteocalcin
Formulation
20 mM TBS, pH7.5, containing 1.0% bovine serum albumin, lyophilized
State of Matter
Lyophilized
Product Preparation
Purified by immunoaffinity chromatography
Storage and Handling
Store stock solution (2. 0 mg/ml) in aliquots at -20 for 1 year, or at 4°C for 6 months after adding 0.1% sodium azide. Avoid repeated freeze-thaw cycles. Diluted antibody should not be stored.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
Sandwich ELISA: 1-10ug/ml with immobilized antigen, pair with catalog no. 50120.

Immunoblotting: 1-10ug/ml, reducing or non-reducing conditions.

Immunohistochemistry: 1-10ug/ml, frozen or paraffin-embedded tissue after proteinase K (0.4m/ml) antigen retrieval.

Endusers should determine optimal concentrations for their applications.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Monoclonal Antibody specific to an epitope within residues 21-31 of human, bovine, rat, rabbit, pig, goat, and sheep Osteocalcin.
Background
Osteocalcin is a noncollagenous, 49 amino acid glutamate-rich polypeptide bone matrix protein with a molecular weight of about 5800Da. Osteoblasts produce osteocalcin and incorporate it into the bone matrix. Osteocalcin is released into the circulation from the matrix during bone resorption and, therefore, is considered a marker of bone turnover rather than a specific marker of bone formation.

Antigen Details

Function
Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
NCBI Gene Bank ID
UniProt.org
Research Area
Hormones/Proteins

References & Citations

Indirect Elisa Protocol
IHC

Formats Available

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Prod No.
Description
50116
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.