Anti-Pan Cytokeratin (Clone AE1/AE3) – Purified No Carrier Protein

Human epidermal keratin

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Liquid

This antibody may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Next Day 2-8°C

IHC
IF
WB
FC

Clone AE1/AE3 activity is directed against cytokeratins.

Keratins belong to the highly conserved intermediate filament family ¹ of water insoluble proteins that form tonofilaments in epidermis and most other epithelia ². More than 20 cytokeratins have been identified, and they are divided into two polypeptide types: acidic (type I) and basic (type II) ^1,3. Cytokeratins are important to epithelial structural integrity and are involved in motility, signaling, growth, and protein synthesis ³. Cytokeratins are also useful markers for epithelial malignancies ^1,3.

Monoclonal antibodies AE1 and AE3 were generated by immunizing BALB/c mice with total keratin isolated from human epidermal callus ². Spleen cells were fused with mouse myeloma cell line P3x63Ag8 to produce hybridomas which were screened for anti-keratin activity by ELISA. Subsequent immunofluorescence staining showed binding to keratin-type intermediate filaments, with AE1 reacting predominantly with epidermal basal cells and AE3 with the entire epidermis (whole layers) ^2,4. Additionally, Western blotting showed AE1 reacts with 48-, 50- and 56-kDa keratins and AE3 reacts with numerous 58- to 67-kDa keratins ².

AE1/AE3 has been used extensively as a pan-cytokeratin cocktail in the pathology setting as well as in multiple species for the study of cellular differentiation. For example, AE1/AE3 stains neoplastic lesions in humans ^5,6,7,8 as well as ameloblasts in monkeys ⁹, some mammary epithelial cells in mouse ¹⁰, myoepithelial cells in guinea pig ¹⁰, the epithelium of normal canine eyes ¹¹, and can be used to study skin graft in cat ¹².

Cytokeratins are present in epithelial cells.

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13. Weiss RA, Guillet GY, Freedberg IM, et al. J Invest Dermatol. 81(3):224-230. 1983.