Anti-Progesterone Receptor (Phospho-Ser190) Antibody (43074)
Data
Immunohistochemistry: Detection of PR (phosphoSer190) in paraffin-embedded
human breast carcinoma tissue.
Immunofluorescence: Detection of PR (phosphoSer190) in methanol-fixed MCF-7
cells.
Immunoblotting:Detection of PR (phosphoSer190) in extracts of SKOV3
cells untreated or treated with
EGF. - -
- -
Antibody DetailsProduct DetailsReactivity Species Human Host Species Rabbit Immunogen Peptide sequence that includes phosphorylation site of Serine 190 (G-L-S(p)-P-A) derived from human progesterone receptor and conjugated to KLH. Product Concentration 1.0 mg/ml Formulation PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. State of Matter Liquid Product Preparation Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide Storage and Handling This antibody is stable for at least one (1) year at -20°C. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~66kDa is detected.
Immunofluorescence: use at dilution of 1:100-1:200. Immunohistochemistry: use at dilution of 1:50-1:100. These are recommended working dilutions. End user should determine optimal dilutions for their applications. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Rabbit Polyclonal Antibody specific to Progesterone Receptor (Phospho-Ser190) Background Progesterone receptor (PR) is a nuclear hormone receptor of the NR3C class which includes mineralocorticoid, glucocorticoid, and androgen receptors. They exist as homodimers coupled to Hsp90 or HMGB proteins which are shed upon activation. Progesterone receptors signal directly via DNA binding and transcriptional regulation of target genes. They can also signal by binding to other proteins with transcription factors such as NF-kappa B, AP-1, or STAT. Progesterone receptor expression is induced by estrogen, and the human gene encoding the progesterone receptor has been localized to 11q22. Antigen DetailsResearch Area Phosphospecific Antibodies References & CitationsWB,IHC,IF |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
