Anti-Rat EphA5

NS0 - Derived Recombinant Rat EphA5 Extracellular Domain

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

Lyophilized

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Next Day Ambient

Immunocytochemistry: This antibody can be used at a concentration of 5-15 µg/mL to detect EphA5 on U-118-MG cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4° C followed by Rhodamine Red-coupled anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA.

Goat Anti-Rat EphA5 recognizes Rat EphA5. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

EPH receptor A5 (EPHA5) is a glycoprotein that is expressed by astrocytes and skeletal muscle. It provides guidance cues for retinal ganglion cells¹ and is essential for proper retinal axon guidance and topographic mapping in the mammalian visual system.² Ephrin A5 serves as a general inhibitor of axonal growth from limbic neurons.3 It also plays a role in motor axon outgrowth and pathfinding to the hindlimb.^4,5

1. Davenport, RW. et al. (1998) J Neurosci. 18: 975
2. Frisen, J. et al. (1998) Neuron 20: 235
3. Gao, PP. et al. (1998) Proceedings of the National Acad of Sci. 95: 5329
4. Eberhart, J. et al. (2000) Developmental Neurosci. 22: 237
5. Eberhart , J. et al. (2004) J of Neurosci. 24: 1070