Anti-Rotavirus (Clone: RV6-25) – Purified No Carrier Protein

Human Mab RV6-25 was sequenced from RV-specific B cells isolated from the blood of healthy adult donors or RV-infected infants or adults^5,6.

This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

Recombinant antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one year. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≥ -70°C. Avoid Repeated Freeze Thaw Cycles.

Standard Overnight on Blue Ice.

ELISA
N
FC

RV6-25 activity is directed against the apical surface of the VP6 head domain. RV6-25 and RV6-26 are encoded by the same heavy chain variable segment⁶, but unlike RV6-26, RV6-25 is unable to inhibit RV⁵. Three-dimensional structural analyses of DLP: Fab complexes show that RV6-25 Fab molecules attach to the tops of VP6 trimers, at one VP6 subunit, with the epitope positioned along the edge and in close contact with the VP6 surface loop⁵. The RV6-25 Fab protrudes from the VP6 trimers and extends away from the viral surface in a regular pattern depending on the position of the VP6 trimer in the VP6 layer¹. Additionally, RV6-25 binds to the apical surface of the DLP VP6 head domain and does not obstruct the transcription pore¹. The Fab binds to epitopes on the distinct P, P′, T, T′, or D VP6 trimers.

Rotaviruses (RV) are double-stranded, non-enveloped, icosahedral RNA viruses in the Reoviridae family¹ that cause severe dehydrating diarrhea in infants and children². RV particles are composed of concentric viral protein (VP) layers³. The triple-layered particle has an inner capsid layer (VP2), an intermediate capsid layer (VP6), and an outer capsid layer (VP7, VP4). The transcriptionally active double-layered particle (DLP) consists of VP2 and VP6. VP6 is the most antigenic RV protein in humans⁴.

This antibody has been tested in lateral flow assays. The best matched pair is RV6-25 (Capture)/RV6-26 (Detection).

1. Aiyegbo MS, Eli IM, Spiller BW, et al. J Virol. 88(1):469-76. 2014.
2. Bern C, Martines J, de Zoysa I, et al. Bull World Health Organ. 70: 705-714. 1992.
3. Pesavento, JB, Crawford SE, Estes MK, et al. Curr Top Microbiol Immunol. 309: 189-219. 2006.
4. McKinney BA, Kallewaard NL, Crowe JE, et al. Immunome Res 3:8. 2007.
5. Kallewaard NL, McKinney BA, Gu Y, et al. J Immunol. 180(6):3980-9. 2008.
6. Weitkamp JH, Kallewaard NL, Bowen AL, et al. J Immunol. 174(6):3454-60. 2005.
7. Weitkamp JH, Kallewaard N, Kusuhara K, et al. J Immunol Methods. 275:223–237. 2003.