Anti-Rotavirus (Clone: RV6-25)

Pricing & Details

Product No.LT582
Clone
RV6-25
Protein
Rotavirus
Formats AvailableView All
Product Type
Recombinant Monoclonal Antibody
Isotype
Human IgG
Applications
ELISA
,
FC
Prod No.
Size
Price
Avail.
Qty
Add to cart
LT582-250 µg
250 µg
$390.00
Pre-order
Max:
Min: 1
Step: 1
LT582-1.0 mg
1.0 mg
$1,400.00
Pre-order
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Rotavirus
Virus
Expression Host
HEK-293
Immunogen
Human Mab RV6-25 was sequenced from RV-specific B cells isolated from the blood of healthy adult donors or RV-infected infants or adults5,6.
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
≤ 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
Formulation
This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one year. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≥ -70°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Standard Overnight on Blue Ice.
Other Applications Reported In Literature ?
ELISA
FC
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
RV6-25 activity is directed against the apical surface of the VP6 head domain. RV6-25 and RV6-26 are encoded by the same heavy chain variable segment6, but unlike RV6-26, RV6-25 is unable to inhibit RV5. Three-dimensional structural analyses of DLP: Fab complexes show that RV6-25 Fab molecules attach to the tops of VP6 trimers, at one VP6 subunit, with the epitope positioned along the edge and in close contact with the VP6 surface loop5. The RV6-25 Fab protrudes from the VP6 trimers and extends away from the viral surface in a regular pattern depending on the position of the VP6 trimer in the VP6 layer1. Additionally, RV6-25 binds to the apical surface of the DLP VP6 head domain and does not obstruct the transcription pore1. The Fab binds to epitopes on the distinct P, P′, T, T′, or D VP6 trimers.
Background
Rotaviruses (RV) are double-stranded, non-enveloped, icosahedral RNA viruses in the Reoviridae family1 that cause severe dehydrating diarrhea in infants and children2. RV particles are composed of concentric viral protein (VP) layers3. The triple-layered particle has an inner capsid layer (VP2), an intermediate capsid layer (VP6), and an outer capsid layer (VP7, VP4). The transcriptionally active double-layered particle (DLP) consists of VP2 and VP6. VP6 is the most antigenic RV protein in humans4.

Antigen Details

Protein
PubMed
Research Area
Infectious Disease
.
Rotavirus
.
Viral

References & Citations

1. Aiyegbo MS, Eli IM, Spiller BW, et al. J Virol. 88(1):469-76. 2014.
2. Bern C, Martines J, de Zoysa I, et al. Bull World Health Organ. 70: 705-714. 1992.
3. Pesavento, JB, Crawford SE, Estes MK, et al. Curr Top Microbiol Immunol. 309: 189-219. 2006.
4. McKinney BA, Kallewaard NL, Crowe JE, et al. Immunome Res 3:8. 2007.
5. Kallewaard NL, McKinney BA, Gu Y, et al. J Immunol. 180(6):3980-9. 2008.
6. Weitkamp JH, Kallewaard NL, Bowen AL, et al. J Immunol. 174(6):3454-60. 2005.
7. Weitkamp JH, Kallewaard N, Kusuhara K, et al. J Immunol Methods. 275:223–237. 2003.
Elisa Sandwich Protocol
Flow Cytometry

Formats Available

Prod No.
Description
LT582
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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