Anti-Rotavirus (Clone: RV6-26)

Product No.: LT581

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Product No.LT581
Clone
RV6-26
Target
Rotavirus
Product Type
Recombinant Monoclonal Antibody
Alternate Names
RV
Isotype
Human IgG
Applications
ELISA
,
FC
,
N

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Product Size
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Antibody Details

Product Details

Reactive Species
Rotavirus
Virus
Expression Host
HEK-293 Cells
Immunogen
Human Mab RV6-26 was sequenced from RV-specific B cells isolated from the blood of healthy adult donors or RV-infected infants or adults5,6.
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
< 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
Formulation
This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Recombinant antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one year. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≥ -70°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Standard Overnight on Blue Ice.
Additional Applications Reported In Literature ?
ELISA
N
FC
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
RV6-26 activity is directed against a quaternary epitope on VP6.

This human monoclonal antibody RV6-26 binds at the viral RNA release pore in the DLP5 and is able to neutralize RV by inhibiting transcription7. Structural data suggest steric hindrance inhibits the transcriptional pore7.
Background
Rotaviruses (RV) are double-stranded, non-enveloped, icosahedral RNA viruses in the Reoviridae family1 that cause severe dehydrating diarrhea in infants and children2. RV particles are composed of concentric viral protein (VP) layers3. The triple-layered particle has an inner capsid layer (VP2), an intermediate capsid layer (VP6), and an outer capsid layer (VP7, VP4). The transcriptionally active double-layered particle (DLP) consists of VP2 and VP6. VP6 is the most antigenic RV protein in humans4. The antibody VH1–46 gene segment is the site of primary interaction with VP65.

Antigen Details

Research Area
Infectious Disease
.
Matched Pair
.
Rotavirus
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Viral

References & Citations

1. Aiyegbo MS, Eli IM, Spiller BW, et al. J Virol. 88(1):469-76. 2014.
2. Bern C, Martines J, de Zoysa I, et al. Bull World Health Organ. 70: 705-714. 1992.
3. Pesavento, JB, Crawford SE, Estes MK, et al. Curr Top Microbiol Immunol. 309: 189-219. 2006.
4. McKinney BA, Kallewaard NL, Crowe JE, et al. Immunome Res 3:8. 2007.
5. Kallewaard NL, McKinney BA, Gu Y, et al. J Immunol. 180(6):3980-9. 2008.
6. Weitkamp JH, Kallewaard N, Kusuhara K, et al. J Immunol Methods. 275:223–237. 2003.
7. Aiyegbo MS, Sapparapu G, Spiller BW, et al. PLoS ONE. 8(5): e61101. 2013.
Indirect Elisa Protocol
Flow Cytometry
N
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Prod No.
Description
LT582

Formats Available

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Prod No.
Description
LT581
Products are for research use only. Not for use in diagnostic or therapeutic procedures.