Anti-RSK1 Reactive with Human, Rat and Mouse

Anti-RSK1 Reactive with Human, Rat and Mouse

Product No.: R1261

[product_table name="All Top" skus="R1261"]

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Target
RPS6K1
Product Type
Polyclonal Antibody
Alternate Names
RSK1, MAPKAPK1a, RPS6KA1
Applications
IHC FFPE
,
WB

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Data

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Antibody Details

Product Details

Reactive Species
Human
Host Species
Rabbit
Immunogen
E. coli-Derived Recombinant Human RSK1
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
State of Matter
Lyophilized
Storage and Handling
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Human RSK1 this polyclonal antibody can be used at a concentration of 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R1190 and a chromogenic substrate such as PN:T343. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Additional Applications Reported In Literature ?
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 5 - 15 µg/ml. The recommended secondary antibody for IHC is PN:R1190. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rabbit Anti-Human Ribosomal Protein S6 Kinase-1 (RSK1) recognizes Human RSK1. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
Ribosomal protein S6 Kinase 1 (RSK1) is a member of the RSK family that consists of growth factor-regulated serine/threonine kinases. RSK1 contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signaling pathway. RSK1 encodes a predicted 735 amino acid protein containing 2 distinct consensus ATP-binding site sequences. RSK1 transcript is present in lymphocytes, skeletal muscle, liver, and adipose tissue. RSKs are implicated in the activation of the mitogen-activated kinase (MAPK) cascade and the stimulation of cell proliferation and differentiation.

Antigen Details

PubMed
NCBI Gene Bank ID
IHC FFPE
General Western Blot Protocol
Products are for research use only. Not for use in diagnostic or therapeutic procedures.