Anti-SARS-CoV-1, Spike (Clone COV1-57) – Purified No Carrier Protein

Anti-SARS-CoV-1, Spike (Clone COV1-57) – Purified No Carrier Protein

Product No.: S1002

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Product No.S1002
Clone
COV1-57
Target
SARS-CoV-1
Spike
Formats AvailableView All
Product Type
Recombinant Monoclonal Antibody
Alternate Names
SARS, S2 protein, Spike glycoprotein 2
Isotype
Human IgG1κ
Applications
N

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Antibody Details

Product Details

Reactive Species
SARS-CoV-1
Expression Host
HEK-293 Cells
Immunogen
Sequenced from PBMCs from a donor who had recovered from a naturally-occurring SARS infection.
Product Concentration
≥1.0 mg/ml
Purity
≥90% monomer by analytical SEC and SDS-Page
Formulation
This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
State of Matter
Liquid
Product Preparation
Recombinant antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Antibodies may be stored sterile as received at 2-8°C for up to one year. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≥ -80°C. Avoid Repeated Freeze Thaw Cycles.
Regulatory Status
Research Use Only
Country of Origin
USA
Shipping
2 – 8° C Wet Ice
Additional Applications Reported In Literature ?
N
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
COV1-57 is highly specific for the SARS-CoV-1 and SARS-CoV-2 prefusion spike protein (S2) distributed on the viral surface.
Background
SARS-CoV-1, also known as the Severe Acute Respiratory Syndrome coronavirus, is a zoonotic virus that is believed to have originated from an animal reservoir, possibly horseshoe bats, and transmitted to humans1. This virus primarily affects the respiratory system and can cause symptoms such as muscle pain, headache, fever, cough, dyspnea, and pneumonia1,2. It was the first severe and readily transmissible new disease to emerge in the 21st century, with the capacity to spread globally via international air travel.

The COV1-57 monoclonal antibody targets the prefusion spike protein of both SARS-CoV-1 and SARS-CoV-2, making it a cross-reactive antibody. Although it does not block the ACE2 receptor, COV1-57 functions as a neutralizing antibody, which means it can inhibit the virus's ability to infect host cells. The neutralizing activity of COV1-57 suggests that it can prevent the spike protein from undergoing the necessary conformational changes required for membrane fusion. The specific epitope targeted by COV1-57 remains unknown, which warrants further research to determine the precise binding site and mechanism of neutralization. This antibody could be valuable in therapeutic applications, especially in combination with other antibodies that target different epitopes.
Antigen Distribution
Anti-SARS-CoV-1 recognizes antigens on infected cells, including respiratory epithelial cells.
NCBI Gene Bank ID
UniProt.org
Research Area
Category C Pathogens
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Infectious Disease
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Viral
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IVD Raw Material

References & Citations

1. Mann R, Perisetti A, Gajendran M, Gandhi Z, Umapathy C, Goyal H. Clinical Characteristics, Diagnosis, and Treatment of Major Coronavirus Outbreaks. Front Med (Lausanne). 2020;7:581521.
2. Rat P, Olivier E, Dutot M. SARS-CoV-2 vs. SARS-CoV-1 management: antibiotics and inflammasome modulators potential. Eur Rev Med Pharmacol Sci. 2020;24(14):7880-7885.

Formats Available

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.