Anti-SRC-1 Antibody (56218)

Product No.: 56218


Clone H4 Target SRC-1 Formats AvailableView All Product Type Monoclonal Alternate Names NCoA-1, EC 2.3.1.48, Class E basic helix-loop-helix protein 74, bHLHe74, Protein Hin-2, RIP160, Renal carcinoma antigen NY-REN-52, Steroid receptor coactivator 1, SRC-1 Isotype Mouse IgG1 Applications WB,IP,ChIP


Select Product Size 100 µg — $587.00	Qty Max: Min: 1 Step: 1 $587.00 ADD TO CART Login For mAb Advantage Pricing Contact Us for Bulk Quantities

Data

Mouse tissue (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with SRC1 antibody [1135] (56218) diluted at 1:2000.


Antibody Details Product Details Reactive Species Human ⋅ Monkey ⋅ Mouse ⋅ Rat Host Species Mouse Immunogen Human SRC-1 (aa 477-947) gst-fusion protein. Product Concentration Lot Specific Formulation 10 mM PBS, pH 7.4. State of Matter Liquid Product Preparation Purified by affinity on GST Storage and Handling This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles. Regulatory Status Research Use Only Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting and Immunoprecipitation: use at 0.5-2 ug/mL. Positive control: COS cells Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity This antibody recognizes human, monkey, mouse, and rat SRC-1. Background SRC-1 (Steroid Receptor Coactivator-1) is a member of a gene family that functions in the full transcriptional activation of the steroid hormone receptor superfamily and contains intrinsic histone acetyltransferase activitiy. The molecular weight of SRC- 1 is 160 kD, and it has a basic helix- loop-helix-PAS domain. Function Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Involved in the coactivation of different nuclear receptors, such as for steroids (PGR, GR and ER), retinoids (RXRs), thyroid hormone (TRs) and prostanoids (PPARs). Also involved in coactivation mediated by STAT3, STAT5A, STAT5B and STAT6 transcription factors. Displays histone acetyltransferase activity toward H3 and H4; the relevance of such activity remains however unclear. Plays a central role in creating multisubunit coactivator complexes that act via remodeling of chromatin, and possibly acts by participating in both chromatin remodeling and recruitment of general transcription factors. Required with NCOA2 to control energy balance between white and brown adipose tissues. Required for mediating steroid hormone response. Isoform 2 has a higher thyroid hormone-dependent transactivation activity than isoform 1 and isoform 3. {PubMed:10449719, PubMed:12954634, PubMed:7481822, PubMed:9223281, PubMed:9223431, PubMed:9296499, PubMed:9427757}. NCBI Gene Bank ID NCOA1 UniProt.org Q15788 Research Area Neuroscience References & Citations View product citations for antibody PRODUCT_CODE on CiteAb Request COA

Antibody Details

Product Details

Reactive Species

Human

⋅

Monkey

⋅

Mouse

⋅

Rat

Host Species

Mouse

Immunogen

Human SRC-1 (aa 477-947) gst-fusion protein.

Product Concentration

Lot Specific

Formulation

10 mM PBS, pH 7.4.

State of Matter

Liquid

Product Preparation

Purified by affinity on GST

Storage and Handling

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.

Regulatory Status

Research Use Only

Country of Origin

USA

Shipping

Next Day 2-8°C

Applications and Recommended Usage?
Quality Tested by Leinco

Immunoblotting and

Immunoprecipitation: use at 0.5-2 ug/mL.

Positive control: COS cells

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

This antibody recognizes human, monkey, mouse, and rat SRC-1.

Background

SRC-1 (Steroid Receptor Coactivator-1) is a member of a gene family that functions in the full transcriptional activation of the steroid hormone receptor superfamily and contains intrinsic histone acetyltransferase activitiy. The molecular weight of SRC- 1 is 160 kD, and it has a basic helix- loop-helix-PAS domain.

Function

Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Involved in the coactivation of different nuclear receptors, such as for steroids (PGR, GR and ER), retinoids (RXRs), thyroid hormone (TRs) and prostanoids (PPARs). Also involved in coactivation mediated by STAT3, STAT5A, STAT5B and STAT6 transcription factors. Displays histone acetyltransferase activity toward H3 and H4; the relevance of such activity remains however unclear. Plays a central role in creating multisubunit coactivator complexes that act via remodeling of chromatin, and possibly acts by participating in both chromatin remodeling and recruitment of general transcription factors. Required with NCOA2 to control energy balance between white and brown adipose tissues. Required for mediating steroid hormone response. Isoform 2 has a higher thyroid hormone-dependent transactivation activity than isoform 1 and isoform 3. {PubMed:10449719, PubMed:12954634, PubMed:7481822, PubMed:9223281, PubMed:9223431, PubMed:9296499, PubMed:9427757}.

NCBI Gene Bank ID

NCOA1

UniProt.org

Q15788

Research Area

Neuroscience