Anti-STAT6 (phospho-Tyr641) Antibody (43050)

Anti-STAT6 (phospho-Tyr641) Antibody (43050)

Product No.: 43050

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Target
STAT6 (phospho-Tyr641)
Product Type
Polyclonal
Isotype
Rabbit
Applications
WB,IHC

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Data

Immunoblotting: Detection of STAT6 (phospho-Tyr641) in extracts of HeLa cells untreated or treated with IL-4.
Immunohistochemistry: Detection of STAT6 (phospho-Tyr641) in paraffin-embedded human breast carcinoma tissue.
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Antibody Details

Product Details

Reactivity Species
Human
Host Species
Rabbit
Immunogen
Peptide sequence that includes phosphorylation site of tyrosine 641 (R-G-Y(p)-V-P) derived from human STAT6 and conjugated to KLH.
Product Concentration
1.0 mg/ml
Formulation
PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
State of Matter
Liquid
Product Preparation
Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide
Storage and Handling
This antibody is stable for at least one (1) year at -20°C.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
Immunoblotting: use at dilution of 1:500-1:1,000. A band of~110kDa is detected.

Immunohistochemistry: use at dilution of 1:50- 1:100.

These are recommended working dilutions.

End user should determine optimal dilutions for their applications.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rabbit Polyclonal Antibody specific to STAT6 (phospho-Tyr641)
Background
Signal transducers and activators of transcription (STATs) are transcription factors that play important roles in signaling pathways induced by many different cytokines and growth factors that regulate cell growth and differentiation, the immune response, antiviral activity, and homeostasis. Their activation and mechanisms of action are examples of direct signaling to the nucleus by extracellular polypeptide ligands without the involvement of second messengers, tyrosine phosphorylation of transcription factors, or transcription factors that make direct contact with membrane-bound receptors and DNA. Since the discovery of STAT 1 and 2, the family has been expanded to seven members.

Antigen Details

Function
Carries out a dual signal transduction and activation of transcription. Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling. {PubMed:17210636}.
NCBI Gene Bank ID
UniProt.org
Research Area
Phosphospecific Antibodies

References & Citations

WB,IHC
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.