Anti-SUR1 Antibody (11567)
Anti-SUR1 Antibody (11567)
Product No.: 11567
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Clone S289-16 Target SUR1 Formats AvailableView All Product Type Monoclonal Alternate Names Sulfonylurea receptor 1 Isotype Mouse IgG1 Applications ICC , IF , IHC , WB |
Data
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SUR1 Monoclonal Antibody, Clone S289-16 (11567). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SUR1 Monoclonal Antibody (11567) at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SUR1 Antibody (D) Composite.
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SUR1 Monoclonal Antibody, Clone S289-16 (11567). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SUR1 Monoclonal Antibody (11567) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) SUR1 Antibody. (D) Composite.
Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa SUR1 protein using Mouse Anti-SUR1 Monoclonal Antibody, Clone S289-16 (11567). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-SUR1 Monoclonal Antibody (11567) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~160 kDa. - -
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Antibody DetailsProduct DetailsReactivity Species Human ⋅ Mouse ⋅ Rat Host Species Mouse Immunogen Fusion protein corresponding to aa 1548-1582 (cytoplasmic C-terminus) of rat SUR1 (accession no. NP_037171.2). Product Concentration Lot Specific Formulation PBS, pH 7.4; 50% glycerol, 0.09% sodium azide. State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at -20°C.
Avoid repeated freeze-thaw cycles.
Regulatory Status For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at 1-2ug/mL. A band of ~160kDa is detected.
Immunohistochemistry: use at 1-10ug/mL. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Mouse brain membranes. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity This antibody recognizes human, mouse, and rat SUR1. It does not cross-react with SUR2B. Background Sulfonylurea receptors (SUR) are membrane proteins that are molecular targets of the sulfonylurea class of antidiabetic drugs whose mechanism of action is to promote insulin release from pancreatic beta cells. SUR proteins are subunits of the inward-rectifier potassium ion channels Kir6.x (6.1 and 6.2). The association of four Kir6.x and four SUR subunits form an ion conducting channel commonly referred to as the KATP channel. The primary function of the SUR is to sense intracellular levels of ATP and ADP and facilitate the opening or closing of its associatedKir6.x potassium channel, thus monitoring the energy balance within cells. Antigen DetailsFunction Subunit of the beta-cell ATP-sensitive potassium channel (KATP). Regulator of ATP-sensitive K(+) channels and insulin release. {UniProtKB:Q09428}. NCBI Gene Bank ID UniProt.org Research Area Ion Channels References & CitationsTechnical ProtocolsICC IF   |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
