Anti-TATA Binding Protein Antibody (70102)
Anti-TATA Binding Protein Antibody (70102)
Product No.: 70102
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Clone 1TBP18 Target TATA Binding Protein Formats AvailableView All Product Type Monoclonal Alternate Names TATA sequence-binding protein, TATA-binding factor, TATA-box factor, Transcription initiation factor TFIID TBP subunit Isotype Mouse IgG1 Applications IP , WB , Gel Shift |
Data
A. Reactivity with rhuTBP and HeLa cell nuclear extract with MAb #70102 at 2ug/ml.B. Epitope mapping by immunoblot with fragments of rhuTBP with MAB #70102 at 2ug/ml.
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Antibody DetailsProduct DetailsReactive Species Human/Mouse/Rat Host Species Mouse Immunogen Recombinant human TBP. Product Concentration Lot Specific Formulation PBS, pH 7.4. State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze- thaw cycles. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco This antibody may be used in Western blot and electrophoretic mobility (gel shift) assays to detect mammalian TBP. It may also be used to immunoprecipitate a truncated TBP molecule containing only the N-terminal domain. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Mouse Monoclonal Antibody that recognizes an epitope within amino acid residues 1-20 of human, mouse and rat TBP. It does not react with TBP from Drosophila, yeast, silk worm, or Xenopus. Other species not investigated. Background The TATA-binding protein (TBP) plays a central role in the assembly of most eukaryotic transcription initiation complexes. TBP assembles with other proteins to form unique multimeric complexes for each of the three different nuclear RNA polymerases. TBP has been cloned from a variety of species and consists of two distinct domains. The C-terminal domain (~180 amino acids) is highly conserved among species, whereas the N-terminal domain varies considerably in length and sequence among different species. The conserved C-terminal domain contains the DNA-binding region as well as regions that interact with positive and negative regulatory proteins. In human TBP, the non-conserved N-terminal domain includes a stretch of glutamine residues ranging from 26 to 42. Several research groups have found an association between expansion of this polyglutamine tract with rare forms of spinocerebellar ataxia and other neurodegenerative diseases including Huntington's disease. Function General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID (PubMed:2374612, PubMed:2363050, PubMed:2194289, PubMed:9836642, PubMed:27193682). Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II (PubMed:2374612, PubMed:2363050, PubMed:2194289, PubMed:9836642, PubMed:27193682). Component of a BRF2-containing transcription factor complex that regulates transcription mediated by RNA polymerase III (PubMed:26638071). Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (pre-initiation complex) during RNA polymerase I-dependent transcription (PubMed:15970593). The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. {PubMed:15970593, PubMed:2194289, PubMed:2363050, PubMed:2374612, PubMed:26638071, PubMed:27193682, PubMed:9836642, ECO:0000305}. NCBI Gene Bank ID UniProt.org Research Area Protein Transcription References & Citations1. Thompson NE et al. 2004. Protein Expression and Purification 36: 186-197. 2. Garcia-Jove Navarro, M., Basset, C., et al. PLoS One . 2013. 8(8):e71443 3. Ribeiro, J. R., Lovasco, L. A., et al. Front Oncol . 2014. 4:45 4. Bouwman, R. D., Palser, A., et al. Retrovirology . 2014. 11:53 5. Leonard, A., Paton, A. W., et al. PLoS One . 2014. 9(10):e110949 6. Kang, K. A., Piao, M. J., et al. Oncotarget. 2016. 7(26):40594-40620 7. Cai, T., Sun, D., et al. J Cell Mol Med. 2018. 22(3):1684-1695 Technical Protocols  Gel Shift Certificate of Analysis |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
