Anti-TGF-β3 (Clone 44922)

Pricing & Details

Product No.T453
Clone
44922
Protein
TGF-β3
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
TGFB3, ARVD, FLJ16571, Transforming Growth Factor Beta 3
Isotype
Mouse
IgG1
Applications
ELISA Cap
,
IHC FFPE
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
T453-25 µg
25 µg
$185.00
In stock
Max:
Min: 1
Step: 1
T453-100 µg
100 µg
$389.00
In stock
Max:
Min: 1
Step: 1
T453-500 µg
500 µg
$920.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human TGF-β3 (>98%)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:T483 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:T198.
Western Blotting: To detect Human TGF-β3 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human TGF-β3 is 20 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:T198.
Other Applications Reported In Literature ?
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 8 µg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human Transforming Growth Factor Beta 3 (TGF-β3) (Clone 44922) recognizes an epitope on Human TGF-β3. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Transforming growth factor-beta 3 (TGF-beta 3) is a member of the TGF beta family of growth factors. It is involved in oxygen-dependent differentiation processes during placental development and pregnancy disorders.1 It also play an important role in wound repair and scarring.2

Antigen Details

Protein
PubMed

References & Citations

1. Mustoe, TA. et al. (1997) Arch Surg. 132: 753
2. Wenger, RH. et al. (2003) Placenta 24: 941
Elisa Sandwich Protocol
IHC
General Western Blot Protocol
Prod No.
Description
T453

Formats Available

Prod No.
Description
T453
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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