Anti-TGF-β3 (Clone 44922)

Product No.: T453


Clone 44922 Target TGF-β3 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names TGFB3, ARVD, FLJ16571, Transforming Growth Factor Beta 3 Isotype IgG1 Applications ELISA Cap , IHC FFPE , WB


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Antibody Details Product Details Reactive Species Human Host Species Mouse Immunogen Purified Recombinant Human TGF-β3 (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831953 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:T483 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:T198. Western Blotting: To detect Human TGF-β3 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human TGF-β3 is 20 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:T198. Additional Applications Reported In Literature ? IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 8 µg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Mouse Anti-Human Transforming Growth Factor Beta 3 (TGF-β3) (Clone 44922) recognizes an epitope on Human TGF-β3. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Transforming growth factor-beta 3 (TGF-beta 3) is a member of the TGF beta family of growth factors. It is involved in oxygen-dependent differentiation processes during placental development and pregnancy disorders.¹ It also play an important role in wound repair and scarring.² PubMed TGF-β3 References & Citations 1. Mustoe, TA. et al. (1997) Arch Surg. 132: 753 2. Wenger, RH. et al. (2003) Placenta 24: 941 View product citations for antibody PRODUCT_CODE on CiteAb Technical Protocols IHC FFPE Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Mouse

Immunogen

Purified Recombinant Human TGF-β3 (>98%)

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2831953

Applications and Recommended Usage?
Quality Tested by Leinco

ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:T483 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:T198.
Western Blotting: To detect Human TGF-β3 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human TGF-β3 is 20 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:T198.

Additional Applications Reported In Literature ?

IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 8 µg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Human Transforming Growth Factor Beta 3 (TGF-β3) (Clone 44922) recognizes an epitope on Human TGF-β3. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background

Transforming growth factor-beta 3 (TGF-beta 3) is a member of the TGF beta family of growth factors. It is involved in oxygen-dependent differentiation processes during placental development and pregnancy disorders.¹ It also play an important role in wound repair and scarring.²

PubMed

TGF-β3

References & Citations

1. Mustoe, TA. et al. (1997) Arch Surg. 132: 753
2. Wenger, RH. et al. (2003) Placenta 24: 941

View product citations for antibody PRODUCT_CODE on CiteAb

IHC FFPE

Certificate of Analysis

Request COA

Prod No.	Description
T453	Anti-TGF-β3 (Clone 44922)

Formats Available

Prod No.	Description
T453	Anti-TGF-β3 (Clone 44922)

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Anti-TGF-β3 (Clone 44922)