Anti-TRAP1/Hsp75 Antibody (11143)
Anti-TRAP1/Hsp75 Antibody (11143)
Product No.: 11143
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Clone 3H4-2H6 Target TRAP1/Hsp75 Formats AvailableView All Product Type Monoclonal Alternate Names HSP 75, TNFR-associated protein 1, Tumor necrosis factor type 1 receptor-associated protein, TRAP-1 Isotype Mouse IgG1 Applications WB |
Data
Immunoblotting: use at 1ug/ml. A band of ~75kDa is detected. Positive control: A431 cell lysate or rat brain lysate. Detection of TRAP1/Hsp75 with #11143 at 1ug/ml. - -
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Antibody DetailsProduct DetailsReactive Species Human ⋅ Rat Host Species Mouse Immunogen Recombinant human TRAP1 Product Concentration 1.0 mg/ml Formulation PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Purified by Protein G affinity chromatography. State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at -20°C. Avoid repeated freeze-thaw cycles. Regulatory Status Research Use Only Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at 1ug/mL. A band of ~75kDa is detected.
Positive control: A431 cell lysate or rat brain lysate. These are recommended concentrations; Enduser should determine optimal concentrations for their applications. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity This antibody recognizes human and rat TRAP1. Other species have not been investigated. Background The molecular chaperone family of 90kDa heat shock proteins (Hsp90) is a highly conserved protein family that plays an important physiological role. Hsp90 is best known for its association with signal transduction processes. Tumor necrosis factor receptor-associated protein 1 (TRAP1) is a recently cloned homolog of Hsp90. Like Hsp90, TRAP1 is associated with numerous proteins that are involved in multiple pathways. Data indicates that TRAP1 is a mitochondrial matrix protein: it is localized in mitochondria of mammalian cells and contains a mitochondrial targeting presequence. Function Chaperone that expresses an ATPase activity. Involved in maintaining mitochondrial function and polarization, downstream of PINK1 and mitochondrial complex I. Is a negative regulator of mitochondrial respiration able to modulate the balance between oxidative phosphorylation and aerobic glycolysis. The impact of TRAP1 on mitochondrial respiration is probably mediated by modulation of mitochondrial SRC and inhibition of SDHA. {PubMed:23525905, PubMed:23564345, PubMed:23747254}. NCBI Gene Bank ID UniProt.org Research Area Heat Shock & Stress Proteins References & CitationsTechnical Protocols Certificate of Analysis |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
