Anti-Versican Antibody (56542)
Anti-Versican Antibody (56542)
Product No.: 56542
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Clone S351-23 Target Versican Formats AvailableView All Product Type Monoclonal Alternate Names Chondroitin sulfate proteoglycan core protein 2, Chondroitin sulfate proteoglycan 2, Large fibroblast proteoglycan, PG-M Isotype Mouse IgG1 Applications ICC , IF , WB |
Data
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Versican Monoclonal Antibody, Clone S351-23 (56542). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Versican Monoclonal Antibody (56542) at 1:200 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Versican Antibody (D) Composite.
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Versican Monoclonal Antibody, Clone S351-23 (56542). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Versican Monoclonal Antibody (56542) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Membrane, Extracellular Space, Extracellular Matrix. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Versican Antibody. (D) Composite.
Western Blot analysis of Rat Brain Membrane and brain showing detection of 350kDa Versican protein using Mouse Anti-Versican Monoclonal Antibody, Clone S351-23 (56542). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane and brain. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Versican Monoclonal Antibody (56542) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: KPL TMB. Predicted/Observed Size: 350kDa. Other Band(s): Multiple other bands. - -
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Antibody DetailsProduct DetailsReactive Species Mouse ⋅ Rat Host Species Mouse Immunogen Fusion protein corresponding to aa 362-585 of mouse Versican (accession no. Q62059). Product Concentration Lot Specific Formulation PBS, pH 7.4; 50% glycerol, 0.09% sodium azide. State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at -20°C. Avoid repeated freezing
and thawing. Regulatory Status For in vitro investigational use only. Not for
use in therapeutic or diagnostic procedures. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at 1ug/mL. Predicted molecular weight is >350kDa.
Positive control: Mouse brain lysate. These are recommended concentrations. User should determine optimal concentrations for their application. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity This antibody recognizes mouse and rat
Versican. Background Versican (chondroitin sulfate proteoglycan 2) is a large extracellular matrix proteo- glycan involved in cell growth and differentiation. Versican contains hyaluronic acid and glycosaminoglycan- binding domains,epidermal growth factor- like repeats, a Lectin-like sequence, and a complement regulatory protein-like domain. Splice variants differ greatly in length and degree of modification by glycoaminoglycan chains. Accumulation around smooth muscle cells in atherosclerotic lesions suggests a role for Versican in atherogenesis. Function May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid. NCBI Gene Bank ID UniProt.org Research Area Neuroscience References & CitationsTechnical ProtocolsICC IF  Certificate of Analysis |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
