Anti-Viral HCMV IL-10 – Biotin

E. coli-Derived Viral HCMV Interleukin-10 (Accession # AAF63437)

This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.

Lyophilized

The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Next Day Ambient

Goat Anti-Human Human Cytomegalovirus Encoded Interleukin 10 (HCMV IL-10) recognizes Human HCMV IL-10. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Human cytomegalovirus (HCMV) is a widespread pathogen that establishes lifelong latent infection facilitated by numerous mechanisms for modulating the host immune system. The UL111A region of the HCMV genome encodes a homolog of human cellular IL-10 (hIL-10). The viral cytokine, cmvIL-10, exhibits many of the immunosuppressive properties of hIL-10. CMVIL-10 has the ability to enhance B cell proliferation.¹ CMV IL10 is expressed in CMV-infected cells and can bind to the human IL10 receptor and can compete with human IL10 for binding sites. CMV IL10 requires both subunits of the IL10 receptor complex to induce signal transduction events and biological activities. CMV IL10 inhibits the proliferation of mitogen-stimulated peripheral blood lymphocytes with the potency of human IL10. It also inhibits cytokine synthesis by these cells. Human CMV IL10 also reduces the cell-surface expression of MHC class 1 and MHC class 2 proteins on peripheral blood mononuclear cells.²

1. Slobedman, B. et al. (2008) Virology 374: 164
2. Spencer, JC. et al. (2002) J Virology 76: 1285