Anti-Zika (ZIKV) E Protein – Purified in vivo GOLD™ Functional Grade

Pricing & Details

Product No.Z200
Clone
ZV67
Protein
ZIKV E (Envelope)
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
ZIKV E, Envelope protein
Isotype
Mouse
IgG2c
Applications
ELISA
,
N
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
Z200-1.0 mg
1.0 mg
$108.00
In stock
Max:
Min: 1
Step: 1
Z200-5.0 mg
5.0 mg
$358.00
In stock
Max:
Min: 1
Step: 1
Z200-25 mg
25 mg
$965.00
In stock
Max:
Min: 1
Step: 1
Z200-50 mg
50 mg
$1,595.00
In stock
Max:
Min: 1
Step: 1
Z200-100 mg
100 mg
$2,300.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Mouse
Immunogen
Injection of a Mouse with ZIKV MR-766, ZIKV H/PF/2013, and ZIKV DIII. 1
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
<1.0 EU/µg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Other Applications Reported In Literature ?
N
ELISA
WB
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone ZV-67 binds to the Zika virus envelope (E) protein at domain III (DIII, LR). 1
Antigen Distribution
The Envelope (E) protein expressed on the Zika Virus
Background
Zika virus (ZIKV) infection during pregnancy is a global public health problem 1, linked causally to severe fetal abnormalities 2. Prophylactic antibodies may prove useful in treating pregnant patients or for designing epitope-specific vaccines 1. The mouse monoclonal antibody (MAb) ZV-67 specifically targets ZIKV and neutralizes infection of the American, Asian, and African strains to varying degrees 1.

ZIKV is a mosquito-transmitted flavivirus that encodes a single polyprotein with an ~11 kb positive-sense RNA open reading frame 1. The polyprotein is cleaved into seven non-structural proteins and three structural proteins (capsid (C), pre-membrane (prM), and envelope (E)). C forms a nucleocapsid. prM complexes with E to facilitate folding and prevent premature fusion to host membranes. E is responsible for viral assembly, attachment, entry, and fusion 1,3 and is a major target of neutralizing antibody research 3. Mature ZIKV virions incorporate 180 copies each of the E and M proteins 4,5.

E is divided into three domains, DI, DII, and DIII 3. DI is a central β-barrel, DII is an extended dimerization domain, and DIII is an immunoglobulin-like segment. The lateral ridge of DIII is targeted by the ZV-67 MAb 1. ZV-67 was generated by priming a lethal mouse model with ZIKV (MR-766 and H/PF/2013) and DIII domain. ZV-67 is of the IgG2c isotype and has been shown to neutralize the MR-766, Uganda 1947, Dakar 41519, and Senegal 1982 African strains as well as the American Paraiba 2015, Brazil strain. It has no cross-reactivity with Japanese Encephalitis or Dengue. Analysis of antibody contact residues by X-ray crystallography shows that ZV-67 binds to the heavy chain complementarity determining region of DIII. A total of 21 residues are contacted by ZV-67, representing four discrete secondary structure elements of the A-strand, B-C, D-E, and F-G loops.

Antigen Details

Research Area
Infectious Disease
.
Viral
.
Zika

References & Citations

1. Zhao H, Fernandez E, Dowd KA. et al. (2016). Cell. 166(4):1016-1027.
2. Brasil P, Pereira Jr JP, Moreira ME. et al. (2016). N Engl J Med. 375(24):2321-2334.
3. Dai L, Song J, Lu X. et al. (2016). 19(5):696-704.
4. Kostyuchenko VA, Lim EX, Zhang S. et al. (2016). Nature. 533(7603):425-428.
5. Sirohi D, Chen Z, Sun L. et al. (2016). Science. 352(6284):467-470.
6. Yi G, Xu X, Abraham S. et al. (2017). EBioMedicine. 25:87-94.
Elisa Sandwich Protocol
General Western Blot Protocol

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.