Artemin is a disulfide-linked homodimeric neurotrophic factor structurally related to GDNF, Neurturin and Persephin. These proteins belong to the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures. Artemin, GDNF, Persephin and Neurturin all signal through a multicomponent receptor system, composed of RET (receptor tyrosine kinase) and one of the four GFRalpha (alpha1-alpha4) receptors. Artemin prefers the receptor GFRalpha3-RET, but will use other receptors as an alternative. Artemin supports the survival of all peripheral ganglia such as sympathetic, neural crest and placodally derived sensory neurons, and dompaminergic midbrain neurons. The functional human Artemin ligand is a disulfide-linked homodimer, of two polypeptide monomers. Each monomer contains seven conserved cysteine residues, one of which is used for interchain disulfide bridging and the others are involved in intramolecular ring formation known as the cysteine knot configuration. In the mature region, human ARTN is 89% and 88% aa identical to rat and mouse ARTN, respectively. It has been reported that Artemin reverses neuropathic pain due to nerve injury, helps resolve morphological changes associated with nerve damage.
>95% by SDS-PAGE and HPLC
<1.0 EU/µg as determined by the LAL method
The biological activity of Human Artemin is determined by its ability to promote survival and neurite outgrowth and dorsal root ganglion neurons.
Protein Accession No.
Amino Acid Sequence
AGGPGSRARA AGARGCRLRS QLVPVRALGL GHRSDELVRF RFCSGSCRRA RSPHDLSLAS LLGAGALRPP PGSRPVSQPC CRPTRYEAVS FMDVNSTWRT VDRLSATACG CLG
State of Matter
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human Artemin is Mr Dimer, 12.0/23.9 kDa (113/226 aa) kDa.
This protein was lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium citrate, 25 mM sodium chloride, pH 4.5
Storage and Stability
The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
Next Day Ambient
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.