Recombinant Human BCA-1

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Pricing & Details

Product. No.B111
Alternate Names
B Cell-Attracting Chemokine-1, CXCL13, BLC, BLR1 Ligand, SCYB13, ANGIE, ANGIE2
Expression Host
E. coli
Species
Human
Prod No.
Size
Price
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Qty
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B111-25 µg
25 µg
$349.00
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Min: 1
Step: 1
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Background

CXCL13, also known as B-lymphocyte chemoattractant (BLC), is a CXC chemokine that is constitutively expressed in secondary lymphoid organs. BCA-1 cDNA encodes a protein of 109 amino acid residues with a leader sequence of 22 residues. Mature human BCA-1 shares 64% amino acid sequence similarity with the mouse protein and 23 - 34% amino acid sequence identity with other known CXC chemokines. Recombinant or chemically synthesized BCA-1 is a potent chemoattractant for B lymphocytes but not T lymphocytes, monocytes or neutrophils. BLR1, a G protein-coupled receptor originally isolated from Burkitt’s lymphoma cells, has now been shown to be the specific receptor for BCA-1. Among cells of the hematopoietic lineages, the expression of BLR1, now designated CXCR5, is restricted to B lymphocytes and a subpopulation of T helper memory cells. Mice lacking BLR1 have been shown to lack inguinal lymph nodes. These mice were also found to have impaired development of Peyer’s patches and defective formation of primary follicles and germinal centers in the spleen as a result of the inability of B lymphocytes to migrate into B cell areas.

Protein Details

Purity
>95% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.01EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human B Cell Attracting Chemokine-1 is determined by its ability to chemoattract human B cells using a concentration of 1.0-10.0 ng/ml.
Expression Host
E. coli
Protein Accession No.
Q53X90
Amino Acid Sequence
vlevyyts lrcrcvqess vfiprrfidr iqilprgngc prkeiivwkk nksivcvdpq aewiqrmmev lrkr
N-terminal Sequence Analysis
Val23
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human BCA-1 is Mr 8.7 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 9 kDa (reducing conditions).
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
PubMed
NCBI Gene Bank
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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