Recombinant Human Cardiac Troponin I Protein

Troponin is a protein complex located along the thin filaments of myofibrils that regulates the contraction of skeletal and cardiac muscles^{1, 2, 3}. It is composed of three subunits: Troponin T (TnT), Troponin C (TnC), and Troponin I (TnI). TnT attaches to tropomyosin to form a Tn-tropomyosin complex on thin filaments. TnC binds to calcium ions, induces conformational changes in TnI, and acts as an anchoring protein of the Tn complex. TnI inhibits actomyosin ATPase.

TnI serves as a biomarker for myocardial infarction (MI)³. During MI, cardiac TnI (cTnI) and TnT (cTnT) are released from injured heart muscles into the bloodstream and their concentrations remain high for several days. The concentration of cTnI rises from ~1-2 ng/mL to ~50 ng/mL within 3–6 hours of MI onset and can reach as high as 500 ng/mL. Since 40% of MI show a normal ECG, and many MI are asymptomatic without apparent chest pain or shortness of breath, precise quantification of cTn levels in a patient’s blood is the “gold standard” for MI diagnosis^{1, 3}. Troponin testing is accomplished by immunoassay, using unique N-terminal peptide sequences that allow for specific detection. A variety of immunoassays utilizing different antibodies and signaling mechanisms have been developed³.

Human

NP_000354.4

MADGSSDAAREPRPAPAPIRRRSSNYRAYATEPHAKKKSKISASRKLQLKTLLLQIAKQELEREAEERRGEKGRALSTRCQPLELAGLGFAELQDLCRQLHARVDKVDEERYDIEAKVTKNITEIADLTQKIFDLRGKFKRPTLRRVRISADAMMQALLGARAKESLDLRAHLKQVKKEDTEKENREVGDWRKNIDALSGMEGRKKKFES

NP

Lyophilized

26k reducing conditions

24k

24k

0.22µm filtered and Lyophilized

0.22µm filtered and Lyophilized

Reconstitute at 1mg/ml using filtered PBS. Gently mix by vortexing and/or inversion for 5 minutes or until fully dissolved. Centrifuge if necessary.

Short Term Storage (Up to one month from date of receipt) - 2 weeks, 2 to 8 °C under sterile conditions after opening and reconstituting. Long Term Storage - 1 year from date of receipt, -20 to -80 °C as supplied.
3 months from date of receipt, -20 to -80 °C sterile conditions after opening and reconstituting.

Ambient

Human

Research Use Only

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2. Katrukha IA. Biochemistry (Mosc). 78(13):1447-1465. 2013.
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4. Järvenpää M-L, Kuningas K, Niemi I, et al. Clin Chim Acta. 414:70–75. 2012.
5. Kar P, Pandey A, Greer JJ, et al. Lab Chip. 12:821. 2012.
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