Recombinant Human CCL23

Chemokine (C-C motif) ligand 23 (CCL23), also known as MPIF-1, is a small cytokine belonging to the CC chemokine family (1). It is most closely related to CCL15. CCL23 is predominantly expressed in lung and liver tissue but is also found in bone marrow, placenta and cells of the myeloid lineage (2). CCL23 induces endothelial cell migration and tube formation. It also enhances the expression of MMP-2 mRNA and protein levels in endothelial cells. CCL23 may play a direct role in angiogenesis (3). It is highly chemotactic for resting T cells and monocytes and slightly chemotactic for neutrophils and has also been attributed to an inhibitory activity on hematopoietic progenitor cells (2). CCL23 has been shown to suppress the low proliferative potential colony-forming cells that give rise to granulocyte and monocyte lineages.

The biological activity of Human MPIF-1 was determined by its ability to induce chemotaxis of human THP-1 cells and mouse BaF/3 cells transfected with hCCR1. The expected ED₅₀ for these effects are typically 5 - 20 ng/ml or 0.70 - 3.5 ng/ml, respectively.

rfhat sadccisytp rsipcslles yfetnsecsk pgvifltkkg rrfcanpsdk qvqvcmrmlk ldtriktrkn

Arg46

Lyophilized

The predicted molecular weight of Recombinant Human CCL23 is Mr 8.6 kDa.

8.6

This recombinant protein was lyophilized from a 0.2 μm filtered solution in 30% acetonitrile (CH₃CN) and 0.1% trifluoroacetic acid (TFA).

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Huang, SA. et al. (2007) J Experimental Hematol. 15: 496
2. Woisetschläger, M. et al. (2007) J Immunol 178: 4335
3. Kim, J. et al. (2006) Biochemical and biophysical res communications 340:498