Recombinant Human CD4 (Soluble)
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BackgroundCD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. CD4 interacts with class II molecules of the major histocompatibility complex (MHC) enhancing the signal for T-cell activation.1 Protein DetailsPurity >90% by SDS-PAGE and analyzed by silver stain. Endotoxin Level <0.1 EU/µg as determined by the LAL method Protein Accession No. Amino Acid Sequence kkvvl gkkgdtvelt ctasqkksiq fhwknsnqik ilgnqgsflt kgpsklndra dsrrslwdqg nfpliiknlk iedsdtyice vedqkeevql lvfgltansd thllqgqslt ltlesppgss psvqcrsprg kniqggktls vsqlelqdsg twtctvlqnq kkvefkidiv vlafqkassi vykkegeqve fsfplaftve kltgsgelww qaerasssks witfdlknke vsvkrvtqdp klqmgkklpl hltlpqalpq yagsgnltla leaktgklhq evnlvvmrat qlqknltcev wgptspklml slklenkeak vskrekavwv lnpeagmwqc llsdsgqvll esnikvlptw N-terminal Sequence Analysis Lys26 State of Matter Lyophilized Predicted Molecular Mass The predicted molecular weight of Recombinant Human Soluble CD4 is Mr 40 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 45 - 48 kDa. Predicted Molecular Mass 40 Formulation This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives. Storage and Stability This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions. Country of Origin USA Shipping Next Day Ambient NCBI Gene Bank Applications and Recommended Usage ? (Quality Tested by Leinco) DISCONTINUED References & Citations1. Hendrickson, WA et al. (1994) Structure 2: 59 Certificate of AnalysisIMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein. |
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