Cardiotrophin-like cytokine (CLC) also known as novel neurotrophin-1/B cell stimulating factor-3 (NNT-1/BSF-3) is a member of the interleukin (IL)-6 family of cytokines.1 CLC is expressed highly in spleen and peripheral leukocytes2 and is a target-derived factor required for the survival of specific pools of motoneurons.3 CLC shows neuroprotective effects on retinal ganglion cells (RGCs) in vivo and might be a treatment option for chronic neurodegenerative eye diseases such as glaucoma.4
>95% by SDS-PAGE and analyzed by silver stain.
<0.01 EU/µg as determined by the LAL method
The biological activity of Human CLC was determined in a cell proliferation assay using a CNTFRα transfected cell line, TF-1.CN5a.1. The expected ED50 for this effect is typically 0.05 - 0.25 μg/ml.
Protein Accession No.
Amino Acid Sequence
lnrgdpgpgp siqktydltr ylehqlrsla gtylnylgpp fnepdfnppr lgaetlprat vdlevwrsln dklrltqnye ayshllcylr glnrqaatae lrrslahfct slqgllgsia gvmaalgypl pqplpgtept wtpgpahsdf lqkmddfwll kelqtwlwrs akdfnrlkkk mqppaaavtl hlgahgf
N-terminal Sequence Analysis
State of Matter
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human CLC is Mr 22.4 kDa.
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 40% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
Next Day Ambient
NCBI Gene Bank
References & Citations
1. Taga, T. et al. (2002) Cytokine 18: 1
2. Shi, Y. et al. (1999) Biochemical Biophysical Res Communications 262: 132
3. Oppenheim, RW. et al. (2003) J Neurosci. 23: 8854
4. Wen, R. et al. (2005) Graefe's Archive for Clinical and Experimental Ophthalmol. 243: 1036
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.