Recombinant Human CLC

Cardiotrophin-like cytokine (CLC) also known as novel neurotrophin-1/B cell stimulating factor-3 (NNT-1/BSF-3) is a member of the interleukin (IL)-6 family of cytokines.¹ CLC is expressed highly in spleen and peripheral leukocytes² and is a target-derived factor required for the survival of specific pools of motoneurons.³ CLC shows neuroprotective effects on retinal ganglion cells (RGCs) in vivo and might be a treatment option for chronic neurodegenerative eye diseases such as glaucoma.⁴

The biological activity of Human CLC was determined in a cell proliferation assay using a CNTFRα transfected cell line, TF-1.CN5a.1. The expected ED₅₀ for this effect is typically 0.05 - 0.25 μg/ml.

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Met

Lyophilized

The predicted molecular weight of Recombinant Human CLC is Mr 22.4 kDa.

22.4

This recombinant protein was lyophilized from a 0.2 μm filtered solution in 40% acetonitrile (CH₃CN) and 0.1% trifluoroacetic acid (TFA).

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Taga, T. et al. (2002) Cytokine 18: 1
2. Shi, Y. et al. (1999) Biochemical Biophysical Res Communications 262: 132
3. Oppenheim, RW. et al. (2003) J Neurosci. 23: 8854
4. Wen, R. et al. (2005) Graefe's Archive for Clinical and Experimental Ophthalmol. 243: 1036