Anti-Human CLC (Clone 138815)

Pricing & Details

Product No.C1462
Clone
138815
Protein
CLC
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Cardiotrophin-Like Cytokine, NR6, B Cell Stimulating Factor-3 (BSF3), Novel Neurotrophin-1 (NNT1), CISS2, CLCF1
Isotype
Mouse
IgG2b
Applications
ELISA Indirect
,
IHC
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C1462-25 µg
25 µg
$185.00
In stock
Max:
Min: 1
Step: 1
C1462-500 µg
500 µg
$513.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
E. coli-Derived Recombinant Human CLC (Accession # Q9UBD9)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as PN:M114. The detection sensitivity of this indirect ELISA for Human CLC is approximately 5 ng/well.
Western Blotting: To detect Human CLC this monoclonal antibody can be used at a concentration of 1 - 2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human CLC is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Other Applications Reported In Literature ?
Immunohistochemistry: Suitable for use at concentration of 8-25 µg/mL.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human Cardiotrophin-Like Cytokine (CLC) (Clone 138815) recognizes an epitope on Human CLC. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Cardiotrophin-like cytokine (CLC) also known as novel neurotrophin-1/B cell stimulating factor-3 (NNT-1/BSF-3) is a member of the interleukin (IL)-6 family of cytokines.1 CLC is expressed highly in spleen and peripheral leukocytes2 and is a target-derived factor required for the survival of specific pools of motoneurons.3 CLC shows neuroprotective effects on retinal ganglion cells (RGCs) in vivo and might be a treatment option for chronic neurodegenerative eye diseases such as glaucoma.4

Antigen Details

Protein
PubMed
NCBI Gene Bank ID

References & Citations

1. Taga, T. et al. (2002) Cytokine 18: 1
2. Shi, Y. et al. (1999) Biochemical Biophysical Res Communications 262: 132
3. Oppenheim, RW. et al. (2003) J Neurosci. 23: 8854
4. Wen, R. et al. (2005) Graefe's Archive for Clinical and Experimental Ophthalmol. 243: 1036
Indirect Elisa Protocol
IHC
General Western Blot Protocol

Formats Available

Prod No.
Description
C1462
Products are for research use only. Not for use in diagnostic or therapeutic procedures.