Purified Recombinant Human CLC (>98%)
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Storage and Handling
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
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Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Human CLC this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Human CLC is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:N138.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Goat Anti-Human Novel Neurotrophin-1 (NNT-1) recognizes Human NNT-1. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Cardiotrophin-like cytokine (CLC) also known as novel neurotrophin-1/B cell stimulating factor-3 (NNT-1/BSF-3) is a member of the interleukin (IL)-6 family of cytokines.1 CLC is expressed highly in spleen and peripheral leukocytes2 and is a target-derived factor required for the survival of specific pools of motoneurons.3 CLC shows neuroprotective effects on retinal ganglion cells (RGCs) in vivo and might be a treatment option for chronic neurodegenerative eye diseases such as glaucoma.4
NCBI Gene Bank ID
References & Citations
1. Taga, T. et al. (2002) Cytokine 18(1):1-7.
2. Shi, Y. et al. (1999) Biochemical Biophysical Res Communications 262:132.
3. Oppenheim, RW. et al. (2003) J Neurosci. 23:8854.
4. Wen, R. et al. (2005) Graefes Arch Clin Exp Ophthalmol. 243(10):1036-42.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.