Recombinant Human CXCL11

Chemokine (C-X-C motif) ligand 11 (CXCL11) is a small cytokine belonging to the CXC chemokine family that is also called Interferon-inducible T-cell alpha chemoattractant (I-TAC) and Interferon-gamma-inducible protein 9 (IP-9). It is highly expressed in peripheral blood leukocytes, pancreas and liver, with moderate levels in thymus, spleen and lung and low expression levels were in small intestine, placenta and prostate.¹ CXCL11 recruits activated Th1 cells to sites of inflammation² and is thought to play a critical role in allograft rejection.³ CXCL11 may also be involved in multiple sclerosis (MS) pathogenesis.²

Measured by its ability to chemoattract human peripheral blood lymphocytes (PBL) cultured in the presence of IL-2 for 21 days. The ED₅₀ for this effect is 10‑20 ng/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR3. The ED₅₀ for this effect is 1‑5 ng/mL.

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Phe22

Lyophilized

The predicted molecular weight of Recombinant Human I-TAC is Mr 8.3 kDa. E. coli-expressed CXCL11/I-TAC preparations are prone to C-terminal proteolytic cleavage. Based on MALDI-TOF mass spectrometry analysis, truncations of three amino acid residues or eleven amino acid residues from the C-terminus may be observed in some preparations. The C-terminal truncation does not appear to affect the bioactivity of the protein

8.3

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives present.

This lyophilized protein is stable for up to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored in the presence of a carrier protein for three months at at -20°C to -70°C without loss of activity in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

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1. Neote, K. et al. (1998) J Exp Med. 187: 2009
2. Losey, J. et al. (2007) Eur Neurol. 58: 228
3. Barr, ML. et al. (2007) Mol Cell Biochem. 296: 1