Anti-Human CXCL11-Biotin

Pricing & Details

Product No.I-277
Protein
CXCL11
Formats AvailableView All
Product Type
Polyclonal Antibody
Alternate Names
Chemokine (C-X-C Motif) Ligand 11, H174, I-TAC, IP-9, IP9, MGC102770, SCYB11, SCYB9B, B-R1
Applications
ELISA Det
,
WB
Prod No.
Size
Price
Avail.
Qty
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I-277-50 µg
50 µg
$525.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Goat
Immunogen
Purified Recombinant Human CXCL11 (>98%)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Storage and Handling
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Human CXCL11 this biotin conjugate can be used at a concentration of 0.1 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Human CXCL11 is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-228.
ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1-0.4 µg/ml when used in conjunction with PN:C1448 as the capture antibody at 2-8 µg/ml and an optimal second step reagent such as PN:A106 for the detection of Human CXCL11.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Anti-Human Interferon-Inducible T-Cell Alpha Chemoattractant (I-TAC) recognizes Human I-TAC. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
Chemokine (C-X-C motif) ligand 11 (CXCL11) is a small cytokine belonging to the CXC chemokine family that is also called Interferon-inducible T-cell alpha chemoattractant (I-TAC) and Interferon-gamma-inducible protein 9 (IP-9). It is highly expressed in peripheral blood leukocytes, pancreas and liver, with moderate levels in thymus, spleen and lung and low expression levels were in small intestine, placenta and prostate.1 CXCL11 recruits activated Th1 cells to sites of inflammation2 and is thought to play a critical role in allograft rejection.3 CXCL11 may also be involved in multiple sclerosis (MS) pathogenesis.2

Antigen Details

Protein
PubMed
NCBI Gene Bank ID

References & Citations

1. Neote, K. et al. (1998) J Exp Med. 187: 2009
2. Losey, J. et al. (2007) Eur Neurol. 58: 228
3. Barr, ML. et al. (2007) Mol Cell Biochem. 296: 1
Indirect Elisa Protocol
General Western Blot Protocol
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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