Purified Recombinant Human CXCL11 (Accession # O146255)
<0.1 EU/µg as determined by the LAL method
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:I-277 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:I-228.
Western Blotting: To detect Human CXCL11 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human CXCL11 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-228.
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Human CXCL11 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human CXCL11 (at 15 ng/ml) is 0.2 - 0.8 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Mouse Anti-Human CXCL11 (Clone 87328) recognizes an epitope on Human CXCL11. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Chemokine (C-X-C motif) ligand 11 (CXCL11) is a small cytokine belonging to the CXC chemokine family that is also called Interferon-inducible T-cell alpha chemoattractant (I-TAC) and Interferon-gamma-inducible protein 9 (IP-9). It is highly expressed in peripheral blood leukocytes, pancreas and liver, with moderate levels in thymus, spleen and lung and low expression levels were in small intestine, placenta and prostate.1 CXCL11 recruits activated Th1 cells to sites of inflammation2 and is thought to play a critical role in allograft rejection.3 CXCL11 may also be involved in multiple sclerosis (MS) pathogenesis.2
NCBI Gene Bank ID
References & Citations
1. Neote, K. et al. (1998) J Exp Med. 187: 2009
2. Losey, J. et al. (2007) Eur Neurol. 58: 228
3. Barr, ML. et al. (2007) Mol Cell Biochem. 296: 1
Products are for research use only. Not for use in diagnostic or therapeutic procedures.