Recombinant Human EGF R

EGF Receptor (EGFR, ErbB1) is a transmembrane protein that exerts tyrosine kinase activity upon ligand induced activation. Those ligands include EGF, TGF-alpha, HB-EGF, BTC, Amphiregulin, Epiregulin, and Epigen (1). When the intrinsic kinase is activated, EGFR tyrosyl-phosphorylates itself and numerous intermediary effector molecules, including closely-related c-erbB receptor family members. This initiates a signaling cascade which includes dimerization and internalization, tyrosine phosphorylation, DNA synthesis of target genes, and cell proliferation (2). In addition to being implicated in organ morphogenesis, maintenance and repair, upregulated EGFR signaling has been correlated in a wide variety of tumors with progression to invasion and metastasis. Thus, EGFR and its downstream signaling molecules are targets for therapeutic interventions in wound repair and cancer (3).

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Leu25

Lyophilized

The predicted molecular weight of Recombinant Human EGF R is Mr 68.6 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE under reducing conditions is Mr 110-115 kDa.

68.6

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) with BSA as a carrier protein. pH 7.2 – 7.3 with BSA.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml.
Flow Cytometry: PN:A106
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CXCR5. A suggested method would be to stain cells expressing CXCR5 with approximately 10 µl per test. A typical test sample constitutes approximately 50 µl of packed whole blood or 1 x 10⁵ continuous passage or activated cell cultures that have been centrifuged at 500 X g for five minutes. Labeling of the cells with the biotin conjugate should be followed by PN:A104, resuspended in 200-400 µl of 1X PBS.

1. Wells, A. et al. (1999) Int. J. Biochem. Cell Biol. 31:637
2. Oda, K. et al. (2005) Mol. Syst. Biol. 1:2005
3. Walker, F. et al. (2009) Human Pathology 40:1517