Recombinant Human Fas Ligand (FasL)
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BackgroundFas ligand (FasL), also known as TNFSF6, is a type II transmembrane protein that belongs to the tumor necrosis factor (TNF) family and is involved in membrane mediated apoptosis. The specific receptor for FasL is Fas (CD95, Apo-1). FasL is primarily expressed on activated T cells and NK cells, while Fas is expressed on various types of cells. Ligation of Fas by FasL triggers activation of the caspase cascade. Functional impairment of the Fas/FasL system is associated with the development and progression of malignancies. Tumor cells frequently exhibit de novo expression of FasL, which plays a significant role in local tissue destruction, metastatic spread and immune escape of the tumor cells.1 The apoptosis of lymphocytes, which occurs in autoimmune diseases, is usually induced by the Fas/FasLsystem, therefore, FasL is regarded as a potential target for immunotherapy.2 One study has suggested a correlation between high levels of FasL expression in the cornea and successful corneal transplantation.3 Protein DetailsPurity >95% by SDS-PAGE and analyzed by silver stain. Endotoxin Level <1.0 EU/µg as determined by the LAL method Biological Activity The biological activity of Human Soluble Fas Ligand is determined by its ability to induce cytotoxicity in Jurkat cells. The expected ED<sub>50</sub> for this effect is <1.0 - 3.0 ng/ml, in the presence of 10 µg/ml of a cross-linking antibody. Fusion Protein Tag Histidine Tag Protein Accession No. Amino Acid Sequence hhhhhhpspp pekkelrkva hltgksnsrs mplewedtyg ivllsgvkyk kgglvinetg lyfvyskvyf rgqscnnlpl shkvymrnsk ypqdlvmmeg kmmsycttgq mwarssylga vfnltsadhl yvnvselslv nfeesqtffg lykl
N-terminal Sequence Analysis Met State of Matter Lyophilized Predicted Molecular Mass The predicted molecular weight of Recombinant Human Fas Ligand is Mr 18 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 19 - 32 kDa (reducing conditions). Predicted Molecular Mass 18 Formulation This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives. Storage and Stability This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions. Country of Origin USA Shipping Next Day Ambient NCBI Gene Bank References & Citations1. Igney, FH. et al. (2005) Cancer Immunol. Immunother. 54:1127 2. Ottmar, J. et al. (2008) Cell Communication and Signaling 6:11 3. Stuart, P. et al. (1997) J. Clin. Investigation 99:396 Certificate of AnalysisIMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein. |
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