Recombinant Human Fas Ligand (FasL)

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Pricing & Details

Product. No.F115
Alternate Names
TNFSF6, CD95L, Apo I Ligand, APTL, FASLG, APT1LG1, CD178
Expression Host
CHO
Species
Human
Prod No.
Size
Price
Avail.
Qty
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F115-10 µg
10 µg
$369.00
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Background

Fas ligand (FasL), also known as TNFSF6, is a type II transmembrane protein that belongs to the tumor necrosis factor (TNF) family and is involved in membrane mediated apoptosis. The specific receptor for FasL is Fas (CD95, Apo-1). FasL is primarily expressed on activated T cells and NK cells, while Fas is expressed on various types of cells. Ligation of Fas by FasL triggers activation of the caspase cascade. Functional impairment of the Fas/FasL system is associated with the development and progression of malignancies. Tumor cells frequently exhibit de novo expression of FasL, which plays a significant role in local tissue destruction, metastatic spread and immune escape of the tumor cells.1 The apoptosis of lymphocytes, which occurs in autoimmune diseases, is usually induced by the Fas/FasLsystem, therefore, FasL is regarded as a potential target for immunotherapy.2 One study has suggested a correlation between high levels of FasL expression in the cornea and successful corneal transplantation.3

Protein Details

Purity
>95% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human Soluble Fas Ligand is determined by its ability to induce cytotoxicity in Jurkat cells. The expected ED50 for this effect is <1.0 - 3.0 ng/ml, in the presence of 10 µg/ml of a cross-linking antibody.
Fusion Protein Tag
Histidine Tag
Expression Host
CHO
Protein Accession No.
Amino Acid Sequence
hhhhhhpspp pekkelrkva hltgksnsrs mplewedtyg ivllsgvkyk kgglvinetg lyfvyskvyf rgqscnnlpl shkvymrnsk ypqdlvmmeg kmmsycttgq mwarssylga vfnltsadhl yvnvselslv nfeesqtffg lykl
N-terminal Sequence Analysis
Met
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human Fas Ligand is Mr 18 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 19 - 32 kDa (reducing conditions).
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
NCBI Gene Bank

References & Citations

1. Igney, FH. et al. (2005) Cancer Immunol. Immunother. 54:1127
2. Ottmar, J. et al. (2008) Cell Communication and Signaling 6:11
3. Stuart, P. et al. (1997) J. Clin. Investigation 99:396
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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