Purified Recombinant Human Fas Ligand (Accession # P48023)
<0.1 EU/µg as determined by the LAL method
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:F164 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:F115.
Flow Cytometry: Concentration: 0.25 µg/106 cells
Other Applications Reported In Literature ?
Neutralization: Measured by its ability to neutralize Fas Ligand/TNFSF6-induced cytotoxicity in the Jurkat human acute T cell leukemia cell line. The Neutralization Dose (ND50) is typically 1-5 ng/mL in the presence of 2 ng/mL Recombinant Human Fas Ligand/TNFSF6 and 10 µg/mL of a cross-linking antibody, Mouse poly-Histidine Monoclonal Antibody.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Mouse Anti-Human Fas Ligand (Clone 100419) recognizes an epitope on Human Fas Ligand. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
NCBI Gene Bank ID
References & Citations
1. Igney, FH. et al. (2005) Cancer Immunol. Immunother. 54:1127
2. Ottmar, J. et al. (2008) Cell Communication and Signaling 6:11
3. Stuart, P. et al. (1997) J. Clin. Investigation 99:396
Products are for research use only. Not for use in diagnostic or therapeutic procedures.