Anti-Human Fas Ligand

Pricing & Details

Product No.F1126
Clone
100419
Protein
Fas Ligand
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
TNFSF6, CD95L, Apo I Ligand, APTL
Isotype
Mouse
IgG2b
Applications
ELISA Cap
,
FC
,
N
Prod No.
Size
Price
Avail.
Qty
Add to cart
F1126-25 µg
25 µg
$205.00
In stock
Max:
Min: 1
Step: 1
F1126-100 µg
100 µg
$431.00
In stock
Max:
Min: 1
Step: 1
F1126-500 µg
500 µg
$917.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human Fas Ligand (Accession # P48023)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:F164 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:F115.
Flow Cytometry: Concentration: 0.25 µg/106 cells
Other Applications Reported In Literature ?
Neutralization: Measured by its ability to neutralize Fas Ligand/TNFSF6-induced cytotoxicity in the Jurkat human acute T cell leukemia cell line. The Neutralization Dose (ND50) is typically 1-5 ng/mL in the presence of 2 ng/mL Recombinant Human Fas Ligand/TNFSF6 and 10 µg/mL of a cross-linking antibody, Mouse poly-Histidine Monoclonal Antibody.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human Fas Ligand (Clone 100419) recognizes an epitope on Human Fas Ligand. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Details

Protein
NCBI Gene Bank ID

References & Citations

1. Igney, FH. et al. (2005) Cancer Immunol. Immunother. 54:1127
2. Ottmar, J. et al. (2008) Cell Communication and Signaling 6:11
3. Stuart, P. et al. (1997) J. Clin. Investigation 99:396
Elisa Sandwich Protocol
Flow Cytometry

Formats Available

Prod No.
Description
F1126
Products are for research use only. Not for use in diagnostic or therapeutic procedures.