Recombinant Mouse Fas Ligand

Fas ligand (FasL) is a type II transmembrane protein that belongs to the tumor necrosis factor (TNF) family. Fas ligand is a homotrimeric protein and signals through trimerization of FasR, which spans the membrane of the "target" cell. This trimerization usually leads to apoptosis, or cell death.

Soluble Fas ligand is generated by cleaving membrane-bound FasL at a conserved cleavage site by the external serine matrix metalloproteinase MMP-7. Soluble FasL is less active than their membrane-bound counterparts and do not induce receptor trimerization and DISC formation. FasL is regarded as a potential target for immunotherapy.¹

Histidine Tag

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Met

Lyophilized

The predicted molecular weight of Recombinant Mouse FasL is Mr 18 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 27-33 kDa.

18

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Igney, FH. et al. (2005) Cancer Immunol. Immunother. 54:1127
2. Ottmar, J. et al. (2008) Cell Communication and Signaling 6:11
3. Stuart, P. et al. (1997) J. Clin. Investigation 99:396