Anti-Mouse Fas Ligand (Clone 101624)

Pricing & Details

Product No.F1074
Clone
101624
Protein
Fas Ligand
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
TNFSF6, CD95L, Apo I Ligand, APTL, FASLG, APT1LG1, CD178
Isotype
Rat
IgG1
Applications
N
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
F1074-25 µg
25 µg
$170.00
In stock
Max:
Min: 1
Step: 1
F1074-500 µg
500 µg
$525.00
In stock
Max:
Min: 1
Step: 1
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Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
NS0-Derived Recombinant Mouse Fas Ligand
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Mouse Fas Ligand this monoclonal antibody can be used at a concentration of 1 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse Fas Ligand is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Mouse Fas Ligand bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse Fas Ligand (at 5 ng/ml) is 10 - 20 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rat Anti-Mouse Fas Ligand (Clone 101624) recognizes an epitope on Mouse Fas Ligand. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Fas ligand (FasL) is a type II transmembrane protein that belongs to the tumor necrosis factor (TNF) family. Fas ligand is a homotrimeric protein and signals through trimerization of FasR, which spans the membrane of the "target" cell. This trimerization usually leads to apoptosis, or cell death.

Soluble Fas ligand is generated by cleaving membrane-bound FasL at a conserved cleavage site by the external serine matrix metalloproteinase MMP-7. Soluble FasL is less active than their membrane-bound counterparts and do not induce receptor trimerization and DISC formation. FasL is regarded as a potential target for immunotherapy.1

Antigen Details

Protein

References & Citations

1. Igney, FH. et al. (2005) Cancer Immunol. Immunother. 54:1127
2. Ottmar, J. et al. (2008) Cell Communication and Signaling 6:11
3. Stuart, P. et al. (1997) J. Clin. Investigation 99:396
General Western Blot Protocol

Formats Available

Prod No.
Description
F1074
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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