Recombinant Human FGF-9

Fibroblast growth factor 9 (glia-activating factor), also known as FGF9 is a glycosylated neurotrophic polypeptide highly expressed in brain.¹ It is a member of the fibroblast growth factor (FGF) family that possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons and may be important for glial cell development. FGF-9 has sequence similarity of approximately 30% to other members of the family of fibroblast growth factors.² It is highly related to FGF-16.
FGF-9 has been shown to mediate its effects by binding to FGF receptors. It efficiently activates the FGFR2c splice form of FGFR2 and the FGFR3b and FGFR3c splice isoforms of FGFR3.³ FGF-9 is a high affinity, heparin dependent ligand for FGFR3 and FGFR2 but not for FGFR1 and FGFR4.⁴

The biological activity of Human FGF-9 was determined by its ability to stimulate proliferation of a mouse fibroblast cell line, Balb/3T3 (Rubin, J.S. et al., 1991, Proc. Natl. Acad. Sci. USA 88:415). The expected ED₅₀ for this effect is typically 1 - 5 ng/ml.

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Met1 & Ala2

Lyophilized

The predicted molecular weight of Recombinant Human FGF-9 is Mr 23 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 22-29 kDa.

23

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Imamura, T. et al. (1999)J Biol Chem 274: 29352
2. Miyamoto, M. et al. (1993)Molecular Cellular Biology 13: 4251
3. Santos-Ocampo, S. et al. (1996)Journal of Biological Chemistry 271: 1726
4. Hecht, D. et al. (1995)Growth Factors 12: 223