Fibroblast growth factor 9 (glia-activating factor), also known as FGF9 is a glycosylated neurotrophic polypeptide highly expressed in brain.1 It is a member of the fibroblast growth factor (FGF) family that possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons and may be important for glial cell development. FGF-9 has sequence similarity of approximately 30% to other members of the family of fibroblast growth factors.2 It is highly related to FGF-16. FGF-9 has been shown to mediate its effects by binding to FGF receptors. It efficiently activates the FGFR2c splice form of FGFR2 and the FGFR3b and FGFR3c splice isoforms of FGFR3.3 FGF-9 is a high affinity, heparin dependent ligand for FGFR3 and FGFR2 but not for FGFR1 and FGFR4.4
>97% by SDS-PAGE and analyzed by silver stain.
<1.0 EU/µg as determined by the LAL method
The biological activity of Human FGF-9 was determined by its ability to stimulate proliferation of a mouse fibroblast cell line, Balb/3T3 (Rubin, J.S. et al., 1991, Proc. Natl. Acad. Sci. USA 88:415). The expected ED50 for this effect is typically 1 - 5 ng/ml.
Sf 21 Insect Cells
Protein Accession No.
Amino Acid Sequence
aplgevgny fgvqdavpfg nvpvlpvdsp vllsdhlgqs eagglprgpa vtdldhlkgi lrrrqlycrt gfhleifpng tiqgtrkdhs rfgilefisi avglvsirgv dsglylgmne kgelygsekl tqecvfreqf eenwyntyss nlykhvdtgr ryyvalnkdg tpregtrtkr hqkfthflpr pvdpdkvpel ykdilsqs
N-terminal Sequence Analysis
Met1 & Ala2
State of Matter
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human FGF-9 is Mr 23 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 22-29 kDa.
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
Next Day Ambient
NCBI Gene Bank
References & Citations
1. Imamura, T. et al. (1999)J Biol Chem 274: 29352
2. Miyamoto, M. et al. (1993)Molecular Cellular Biology 13: 4251
3. Santos-Ocampo, S. et al. (1996)Journal of Biological Chemistry 271: 1726
4. Hecht, D. et al. (1995)Growth Factors 12: 223
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.