Recombinant Human IL-17A/F Heterodimer

Recombinant Human IL-17A/F Heterodimer

Product No.: I-844

[product_table name="All Top" skus="I-844"]

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Alternate Names
Product Type
Recombinant Protein
Expression Host
E. coli Cells

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Select Product Size
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Protein Details

>95% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.01 EU/µg as determined by the LAL method
Protein Accession No.
Q16552 (IL-17A) & Q96PD4 (IL-17F)
Amino Acid Sequence
(Human IL-17A) mlglaflsea aarkipkvgh tffqkpescp pvpggsmkld igiinenqrv smsrniesrs tspwnytvtw dpnrypsevv qaqcrnlgci naqgkedism nsvpiqqetl vvrrkhqgcs vsfqlekvlv tvgctcv (Human IL-17F)rkipkvghtf fqkpescppv pggsmkldig iinenqrvsm srniesrsts pwnytvtwdp nrypsevvqa qcrnlgcina qgkedismns vpiqqetlvv rrkhqgcsvs fqlekvlvtv gctcvtpvih hvq
N-terminal Sequence Analysis
State of Matter
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human IL-17A/F is Mr IL17A: 15.7; IL17F: 15 kDa.
Predicted Molecular Mass
IL17A: 15.7; IL17F: 15
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
Next Day Ambient
NCBI Gene Bank
Applications and Recommended Usage ?
(Quality Tested by Leinco)
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml.
Flow Cytometry: PN:A106
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CXCR5. A suggested method would be to stain cells expressing CXCR5 with approximately 10 µl per test. A typical test sample constitutes approximately 50 µl of packed whole blood or 1 x 105 continuous passage or activated cell cultures that have been centrifuged at 500 X g for five minutes. Labeling of the cells with the biotin conjugate should be followed by PN:A104, resuspended in 200-400 µl of 1X PBS.
Elisa Sandwich Protocol
Flow Cytometry
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
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Prod No.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.