Recombinant Human IL-28B

r lrgalpdarg chiaqfksls pqelqafkra kdaleeslll kdckcrsrlf prtwdlrqlq vrerpvalea elaltlkvle atadtdpalg dvldqplhtl hhilsqlrac iqpqptagpr trgrlhhwlh rlqeapkkes pgcleasvtf nlfrlltrdl ncvasgdlcv hhhhhh

Lyophilized

The predicted molecular weight of Recombinant Human IL-28B is Mr 20.1 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 22 kDa.

20.1

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) and sodium chloride (NaCl) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml.
Flow Cytometry: PN:A106
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CXCR5. A suggested method would be to stain cells expressing CXCR5 with approximately 10 µl per test. A typical test sample constitutes approximately 50 µl of packed whole blood or 1 x 10⁵ continuous passage or activated cell cultures that have been centrifuged at 500 X g for five minutes. Labeling of the cells with the biotin conjugate should be followed by PN:A104, resuspended in 200-400 µl of 1X PBS.