Recombinant Human IL-7

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Pricing & Details

Product. No.I-189
Alternate Names
Interleukin-7 Lymphopoietin 1, LP-1, Pre-B Cell Factor
Expression Host
E. coli
Species
Human
Prod No.
Size
Price
Avail.
Qty
Add to cart
I-189-5 µg
5 µg
$239.00
In stock
Max:
Min: 1
Step: 1
I-189-10 µg
10 µg
$299.00
In stock
Max:
Min: 1
Step: 1
I-189-25 µg
25 µg
$729.00
In stock
Max:
Min: 1
Step: 1

Background

Interleukin 7 (IL-7), also known as Lymphopoietin 1, is a hematopoietic growth factor which affects primarily early B and T cells (1). It stimulates the differentiation of multipotent hematopoietic stem cells into lymphoid progenitor cells. IL-7 is important for proliferation during certain stages of B cell maturation, T and NK cell survival, development and homeostasis. Produced by thymic stromal cells, spleen cells and keratinocytes, IL-7 can also co-stimulate the proliferation of mature T cells in combination with other factors such as ConA and IL-2 (2). In myeloid lineage cells, IL-7 up-regulates the production of pro-inflammatory cytokines and stimulates the tumoricidal activity of monocytes and macrophages. IL-7 has significant clinical and biomedical potential, such as cancer therapy and HIV infections (3). Human and murine IL-7 is cross-species reactive.

Protein Details

Purity
>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.01EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human IL-7 was Measured as its T cell growth factor activity in a cell proliferation assay using PHA activated human peripheral blood lymphocytes (Yokota, T. et al., 1986, Proc. Nat. Acad. Sci. USA 88:5894). The expected ED50 for this effect is typically 0.2 - 0.5 ng/ml.
Expression Host
E. coli
Protein Accession No.
Amino Acid Sequence
mdcdie gkdgkqyesv lmvsidqlld smkeigsncl nnefnffkrh icdankegmf lfraarklrq flkmnstgdf dlhllkvseg ttillnctgq vkgrkpaalg eaqptkslee nkslkeqkkl ndlcflkrll qeiktcwnki lmgtkeh
N-terminal Sequence Analysis
Met
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human IL-7 is Mr 17 kDa.
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 with no calcium, magnesium, or preservatives present.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
PubMed
NCBI Gene Bank

References & Citations

1. Namen, AE. et al. (1990) Int. J. Cell Cloning 1:168
2. Reed, SG. et al. (1989) J. Exp. Med. 169:707
3. Prakash, S. et al. (2008) Appl. of Biochem. Biotechnol. 151:93
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.