Antibody DetailsProduct DetailsReactivity Species Mouse Host Species Goat Immunogen Purified Recombinant Mouse IL-7 (Accession No.: Q544C8) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830560 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 0.8 µg/ml. A suitable detection antibody is PN:I-259 at a concentration of approximately 0.2 0.8 µg/ml. A suggested standard for this assay is PN:I-533. Western Blotting: To detect Mouse IL-7 this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Mouse IL-7 is 5.0 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-533. Other Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Mouse IL-7 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse IL-7 (at 1.5 ng/ml) is 0.2 - 0.4 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Rabbit Anti-Mouse Interleukin-7 (IL-7) recognizes Mouse IL-7. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Interleukin 7 (IL-7) is a glycoprotein cytokine initially referred to as pre-B cell growth factor because of its growth-promoting activity for cells early in the B lymphocyte lineage. Subsequent to IL-7 gene cloning and expression, IL-7 was also found to influence the activity of cells in the T lineage.1 IL-7 stimulates the differentiation of multipotent (pluripotent) hematopoietic stem cells into lymphoid progenitor cells. It also stimulates proliferation of all cells in the lymphoid lineage (B and T lymphocytes and NK cells). IL-7 is not only a potent grown stimulus for pre-B cells, but also plays a role in T cell activation.2 IL-7 has significant clinical and biomedical potential, such as cancer therapy and HIV infections.3 Antigen DetailsProtein PubMed NCBI Gene Bank ID UniProt.org References & Citations1. Namen, AE. et al. (1990) Int J Cell Cloning 1: 168 2. Reed, SG. et al. (1989) J Exp Med. 169: 707 3. Prakash, S. et al. (2008) Appl. of Biochem Biotechnol. 151: 93 Technical Protocols  |
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