Purified Recombinant Human IL-7 (Accession # P13232)
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
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Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2 µg/ml. A suitable detection antibody is PN:I-258 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:I-189.
Western Blotting: To detect Human IL-7 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human IL-7 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-189.
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Human IL-7 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human IL-7 (at 2.5 ng/ml) is 0.4 - 0.8 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Mouse Anti-Human Interleukin-7 (IL-7) recognizes Human IL-7. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
IL-7 is a glycoprotein cytokine initially referred to as pre-B cell growth factor because of its growth-promoting activity for cells early in the B lymphocyte lineage. Subsequent to IL-7 gene cloning and expression, IL-7 was also found to influence the activity of cells in the T lineage.1 IL-7 stimulates the differentiation of multipotent (pluripotent) hematopoietic stem cells into lymphoid progenitor cells. It also stimulates proliferation of all cells in the lymphoid lineage (B and T lymphocytes and NK cells). IL-7 is not only a potent grown stimulus for pre-B cells, but also plays a role in T cell activation.2 IL-7 has significant clinical and biomedical potential, such as cancer therapy and HIV infections.3
References & Citations
1. Namen, AE. et al. (1990) Int J Cell Cloning 1: 168
2. Reed, SG. et al. (1989) J Exp Med. 169: 707
3. Prakash, S. et al. (2008) Appl. of Biochem Biotechnol. 151: 93
Products are for research use only. Not for use in diagnostic or therapeutic procedures.