Recombinant Human M-CSF

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Pricing & Details

Product. No.M155
Alternate Names
CSF-1, MGI-IM, MGC31930
Expression Host
E. coli
Species
Human
Prod No.
Size
Price
Avail.
Qty
Add to cart
M155-5 µg
5 µg
$169.00
In stock
Max:
Min: 1
Step: 1
M155-10 µg
10 µg
$239.00
In stock
Max:
Min: 1
Step: 1
M155-25 µg
25 µg
$525.00
In stock
Max:
Min: 1
Step: 1

Data

Background

Macrophage colony-stimulating factor (M-CSF), also known as CSF-1, is a secreted cytokine and key regulator of cellular proliferation, differentiation and survival of blood monocytes, tissue macrophages and their progenitor cells. It is produced by monocytes, fibroblasts and endothelial cells and binds to the CSF-1 receptor, also known as CD115. It enhances cytotoxicity, superoxide production, phagocytosis, chemotaxis and secondary cytokine production in monocytes and macrophages (1). M-CSF also primes macrophage killing of tumor cells and microorganisms and stimulates pinocytosis. It is known as one of the factors essential for osteoclast development (2). M-CSF has been shown to play important roles in modulating dermal thickness and growth of the decidua and placenta during pregnancy (3). It is clinically used in the treatment of infection, malignancies and atherosclerosis, as well as, facilitating hematopoietic recovery after bone marrow transplantation (4).

Protein Details

Purity
>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human M-CSF was determined in a cell proliferation assay using an M-CSF-dependent murine monocytic cell line, M-NFS-60 (Halenbeck, R. et al., 1989, Biotechnology 7:710 - 715). The expected ED50 for this effect is 0.5 - 1.5 ng/ml.
Expression Host
E. coli
Protein Accession No.
Amino Acid Sequence
meevseycs hmigsghlqs lqrlidsqme tscqitfefv dqeqlkdpvc ylkkafllvq dimedtmrfr dntpnaiaiv qlqelslrlk scftkdyeeh dkacvrtfye tplqllekvk nvfnetknll dkdwnifskn cnnsfaecss qgherqsegs
N-terminal Sequence Analysis
Met
State of Matter
Solution
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human M-CSF is Mr 18.5 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 37 kDa.
Formulation
This recombinant protein solution was 0.2 µm filtered and formulated in modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives present.
Storage and Stability
Working aliquots of this recombinant protein solution are stable for up to twelve months at -20°C to -70°C in a manual defrost freezer. Upon thawing, in the presence of a carrier protein, this recombinant protein can be stored at 2° - 8°C for one month without detectable loss of activity. For long-term storage, aliquot and freeze at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles.
Country of Origin
USA
Shipping
Dry Ice
PubMed
NCBI Gene Bank

References & Citations

1. Nemunaitis, J. et al. (1998) Clin. Infect. Dis. 26:1279
2. Ko, JS. et al. (2002) Exp. Mol. Med. 34:340
3. Makrigiannakis, A. et al. (2006) Trends Endicrinol. Metab. 17:178
4. Van de Pol, CJ. et al. (1991) Biotechnol. Therap. 2:231
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.