Anti-Human M-CSF (Clone 26730)

Anti-Human M-CSF (Clone 26730)

Product No.: M1269

[product_table name="All Top" skus="M1269"]

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Clone
26730
Target
M-CSF
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
CSF-1, MGI-IM, MGC31930
Isotype
IgG2a
Applications
N
,
WB

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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human M-CSF
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Human M-CSF this monoclonal antibody can be used at a concentration of 1 - 2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human M-CSF is 20 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:M155.
Additional Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Human M-CSF bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human M-CSF (at 2.5 ng/ml) is 0.005 - 0.02 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
Mouse Anti-Human macrophage colony stimulating factor (M-CSF) (Clone 26730) recognizes an epitope on Human M-CSF. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody does not cross-react with rmM-CSF
Background
Macrophage colony-stimulating factor, or M-CSF, is a secreted cytokine which influences hemopoietic stem cells to differentiate into macrophages or other related cell types. M-CSF is a key regulator of cellular proliferation, differentiation, and survival of blood monocytes, tissue macrophages and their progenitor cells. It enhances cytotoxicity, superoxide production, phagocytosis, chemotaxis, and secondary cytokine production in monocytes and macrophages.1 It binds to the Colony stimulating factor 1 receptor. M-CSF is known as one of the factors essential for osteoclast development.2
PubMed
Research Area
Other Molecules

References & Citations

1. Nemunaitis, J. et al. (1998) Clin. Infect. Dis. 26: 1279
2. Ko, JS. et al. (2002) Exp Mol Med 34: 340
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General Western Blot Protocol

Certificate of Analysis

Formats Available

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Prod No.
Description
M1269
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.