Anti-Human M-CSF (Clone 26730)

Pricing & Details


Product No.M1269 Clone 26730 Protein M-CSF Formats AvailableView All Product Type Monoclonal Antibody Alternate Names CSF-1, MGI-IM, MGC31930 Isotype Mouse IgG2a Applications N , WB Submit A Review	Printable Datasheet Printable SDS

Prod No.	Size	Price	Avail.	Qty	Add to cart
M1269-25 µg	25 µg	$225.00	In stock	Max: Min: 1 Step: 1	Add to Cart
M1269-100 µg	100 µg	$430.00	In stock	Max: Min: 1 Step: 1	Add to Cart
M1269-500 µg	500 µg	$1,025.00	In stock	Max: Min: 1 Step: 1	Add to Cart


Antibody Details Product Details Reactivity Species Human Host Species Mouse Immunogen Purified Recombinant Human M-CSF Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831262 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human M-CSF this monoclonal antibody can be used at a concentration of 1 - 2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human M-CSF is 20 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:M155. Other Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Human M-CSF bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human M-CSF (at 2.5 ng/ml) is 0.005 - 0.02 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Specificity Mouse Anti-Human macrophage colony stimulating factor (M-CSF) (Clone 26730) recognizes an epitope on Human M-CSF. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody does not cross-react with rmM-CSF Background Macrophage colony-stimulating factor, or M-CSF, is a secreted cytokine which influences hemopoietic stem cells to differentiate into macrophages or other related cell types. M-CSF is a key regulator of cellular proliferation, differentiation, and survival of blood monocytes, tissue macrophages and their progenitor cells. It enhances cytotoxicity, superoxide production, phagocytosis, chemotaxis, and secondary cytokine production in monocytes and macrophages.¹ It binds to the Colony stimulating factor 1 receptor. M-CSF is known as one of the factors essential for osteoclast development.² Antigen Details Protein M-CSF PubMed M-CSF References & Citations 1. Nemunaitis, J. et al. (1998) Clin. Infect. Dis. 26: 1279 2. Ko, JS. et al. (2002) Exp Mol Med 34: 340 Technical Protocols

Antibody Details

Product Details

Reactivity Species

Human

Host Species

Mouse

Immunogen

Purified Recombinant Human M-CSF

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2831262

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Human M-CSF this monoclonal antibody can be used at a concentration of 1 - 2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human M-CSF is 20 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:M155.

Other Applications Reported In Literature ?

Neutralization: This antibody is useful for neutralization of Human M-CSF bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human M-CSF (at 2.5 ng/ml) is 0.005 - 0.02 µg/ml.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Human macrophage colony stimulating factor (M-CSF) (Clone 26730) recognizes an epitope on Human M-CSF. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody does not cross-react with rmM-CSF

Background

Macrophage colony-stimulating factor, or M-CSF, is a secreted cytokine which influences hemopoietic stem cells to differentiate into macrophages or other related cell types. M-CSF is a key regulator of cellular proliferation, differentiation, and survival of blood monocytes, tissue macrophages and their progenitor cells. It enhances cytotoxicity, superoxide production, phagocytosis, chemotaxis, and secondary cytokine production in monocytes and macrophages.¹ It binds to the Colony stimulating factor 1 receptor. M-CSF is known as one of the factors essential for osteoclast development.²

Antigen Details

Protein

M-CSF

PubMed

M-CSF

References & Citations

1. Nemunaitis, J. et al. (1998) Clin. Infect. Dis. 26: 1279
2. Ko, JS. et al. (2002) Exp Mol Med 34: 340

Prod No.	Description
M1276	Anti-Mouse M-CSF (Clone 131621)
M1269	Anti-Human M-CSF (Clone 26730)
M141	Anti-Human M-CSF
M155	Recombinant Human M-CSF
M1227	Recombinant Rat M-CSF
M1098	Recombinant Mouse M-CSF
M140	Anti-Human M-CSF – Biotin
M1102	Anti-Mouse M-CSF – Biotin
M1324	Mouse M-CSF ELISA Development Kit

Formats Available

Prod No.	Description
M1269	Anti-Human M-CSF (Clone 26730)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.