Recombinant Mouse M-CSF

Recombinant Mouse M-CSF

Product No.: M1098

[product_table name="All Top" skus="M1098"]

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Alternate Names
CSF-1, MGI-IM, MGC31930, Csfm, C87615
Product Type
Recombinant Protein
Expression Host
E. coli Cells
Species
Mouse

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Product Size
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Background

Macrophage colony-stimulating factor, or M-CSF, is a secreted cytokine which influences hemopoietic stem cells to differentiate into macrophages or other related cell types. M-CSF is a key regulator of cellular proliferation, differentiation, and survival of blood monocytes, tissue macrophages and their progenitor cells. It enhances cytotoxicity, superoxide production, phagocytosis, chemotaxis, and secondary cytokine production in monocytes and macrophages.1 It binds to the Colony stimulating factor 1 receptor. M-CSF is known as one of the factors essential for osteoclast development.2

Protein Details

Purity
>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Mouse M-CSF was determined by a cell proliferation assay using an M-CSF-dependent murine monocytic cell line, M-NFS-60 (Halenbeck, R. et al., 1989, Biotechnology 7:710 - 715). The expected ED<sub>50</sub> for this effect is typically 0.5 - 1.5 ng/ml.
Protein Accession No.
Amino Acid Sequence
mkevsehcs hmignghlkv lqqlidsqme tscqiafefv dqeqlddpvc ylkkafflvq diidetmrfk dntpnanate rlqelsnnln scftkdyeeq nkacvrtfhe tplqllekik nffnetknll ekdwniftkn cnnsfakcss rdvvtkpdcn clypkatpss dpasasphqp papsmaplag lawddsqrte gssllpselp lriedpgsak qrpprstcqt le
N-terminal Sequence Analysis
Met
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Mouse M-CSF is Mr 26 kDa. However, the actual molecular weight of the biologically active disulfide-linked homodimeric protein as observed by migration on SDS-PAGE is Mr 29 kDa.
Predicted Molecular Mass
26
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient

References & Citations

1. Nemunaitis, J. et al. (1998) Clin. Infect. Dis. 26: 1279
2. Ko, JS. et al. (2002) Exp Mol Med 34: 340
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.