Anti-Mouse M-CSF (Clone 131621)

Anti-Mouse M-CSF (Clone 131621)

Product No.: M1276

[product_table name="All Top" skus="M1276"]

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Clone
131621
Target
M-CSF
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
CSF-1, MGI-IM, MGC31930
Isotype
IgG2b
Applications
ELISA Cap
,
N

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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rat
Immunogen
Purified Recombinant Mouse M-CSF (>98%)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:M1102 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:M1098.
Additional Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Mouse M-CSF bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse M-CSF (at 2.5 ng/ml) is 4 - 16 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rat Anti-Mouse macrophage colony stimulating factor (M-CSF) (Clone 131621) recognizes an epitope on Mouse M-CSF. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Macrophage colony-stimulating factor, or M-CSF, is a secreted cytokine which influences hemopoietic stem cells to differentiate into macrophages or other related cell types. M-CSF is a key regulator of cellular proliferation, differentiation, and survival of blood monocytes, tissue macrophages and their progenitor cells. It enhances cytotoxicity, superoxide production, phagocytosis, chemotaxis, and secondary cytokine production in monocytes and macrophages.1 It binds to the Colony stimulating factor 1 receptor. M-CSF is known as one of the factors essential for osteoclast development.2

Antigen Details

PubMed
Research Area
Other Molecules

References & Citations

1. Nemunaitis, J. et al. (1998) Clin. Infect. Dis. 26: 1279
2. Ko, JS. et al. (2002) Exp Mol Med 34: 340
Elisa Sandwich Protocol
N

Certificate of Analysis

Formats Available

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Prod No.
Description
M1276
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.